Genome Transplantation in Bacteria: Changing One Species to Another

by: Carole Lartigue, John I Glass, Nina Alperovich, Rembert Pieper, Prashanth P Parmar, Hutchison, Hamilton O Smith, Craig J Venter

Science (28 June 2007), 1144622.

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Abstract

As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol-mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria. 10.1126/science.1144622

@article{citeulike:1424467, abstract = {As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol-mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria. 10.1126/science.1144622}, author = {Lartigue, Carole and Glass, John I. and Alperovich, Nina and Pieper, Rembert and Parmar, Prashanth P. and Hutchison and Smith, Hamilton O. and Venter, Craig J. }, citeulike-article-id = {1424467}, doi = {http://dx.doi.org/10.1126/science.1144622}, journal = {Science}, keywords = {bacterial, genome, specy}, month = {June}, pages = {1144622+}, posted-at = {2007-08-09 15:36:57}, priority = {2}, title = {Genome Transplantation in Bacteria: Changing One Species to Another}, url = {http://dx.doi.org/10.1126/science.1144622}, year = {2007} }

RIS record

TY - JOUR ID - citeulike:1424467 L3 - citeulike-article-id:1424467 TI - Genome Transplantation in Bacteria: Changing One Species to Another JF - Science SP - 1144622 N2 - As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol-mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria. 10.1126/science.1144622 KW - bacterial KW - genome KW - specy AU - Lartigue, Carole AU - Glass, John AU - Alperovich, Nina AU - Pieper, Rembert AU - Parmar, Prashanth AU - Hutchison AU - Smith, Hamilton AU - Venter, Craig PY - 2007/06/28/ UR - http://dx.doi.org/10.1126/science.1144622 ER -

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