Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories

@article{citeulike:1847020, abstract = {Organization in biological membranes spans many orders of magnitude in length scale, but limited resolution in far-field light microscopy has impeded distinction between numerous biomembrane models. One canonical example of a heterogeneously distributed membrane protein is hemagglutinin (HA) from influenza virus, which is associated with controversial cholesterol-rich lipid rafts. Using fluorescence photoactivation localization microscopy, we are able to image distributions of tens of thousands of HA molecules with subdiffraction resolution ({approx}40 nm) in live and fixed fibroblasts. HA molecules form irregular clusters on length scales from {approx}40 nm up to many micrometers, consistent with results from electron microscopy. In live cells, the dynamics of HA molecules within clusters is observed and quantified to determine an effective diffusion coefficient. The results are interpreted in terms of several established models of biological membranes. 10.1073/pnas.0708066104}, author = {Hess, Samuel T. and Gould, Travis J. and Gudheti, Manasa V. and Maas, Sarah A. and Mills, Kevin D. and Zimmerberg, Joshua }, citeulike-article-id = {1847020}, doi = {10.1073/pnas.0708066104}, journal = {Proceedings of the National Academy of Sciences}, keywords = {fpalm, imaging, raft, single\_molecule}, month = {October}, number = {44}, pages = {17370--17375}, posted-at = {2007-11-09 23:16:20}, priority = {2}, title = {Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories}, url = {http://dx.doi.org/10.1073/pnas.0708066104}, volume = {104}, year = {2007} }

TY - JOUR ID - citeulike:1847020 L3 - citeulike-article-id:1847020 TI - Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories JF - Proceedings of the National Academy of Sciences VL - 104 IS - 44 SP - 17370 EP - 17375 N2 - Organization in biological membranes spans many orders of magnitude in length scale, but limited resolution in far-field light microscopy has impeded distinction between numerous biomembrane models. One canonical example of a heterogeneously distributed membrane protein is hemagglutinin (HA) from influenza virus, which is associated with controversial cholesterol-rich lipid rafts. Using fluorescence photoactivation localization microscopy, we are able to image distributions of tens of thousands of HA molecules with subdiffraction resolution ({approx}40 nm) in live and fixed fibroblasts. HA molecules form irregular clusters on length scales from {approx}40 nm up to many micrometers, consistent with results from electron microscopy. In live cells, the dynamics of HA molecules within clusters is observed and quantified to determine an effective diffusion coefficient. The results are interpreted in terms of several established models of biological membranes. 10.1073/pnas.0708066104 KW - fpalm KW - imaging KW - raft KW - single_molecule AU - Hess, Samuel AU - Gould, Travis AU - Gudheti, Manasa AU - Maas, Sarah AU - Mills, Kevin AU - Zimmerberg, Joshua PY - 2007/10/30/ UR - http://dx.doi.org/10.1073/pnas.0708066104 ER -