Induction of pluripotent stem cells from adult human fibroblasts by defined factors.

@article{citeulike:2021374, abstract = {Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts.}, address = {Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.}, author = {Takahashi, K. and Tanabe, K. and Ohnuki, M. and Narita, M. and Ichisaka, T. and Tomoda, K. and Yamanaka, S. }, citeulike-article-id = {2021374}, doi = {10.1016/j.cell.2007.11.019}, issn = {0092-8674}, journal = {Cell}, keywords = {biomedical}, month = {November}, number = {5}, pages = {861--872}, posted-at = {2008-01-29 16:04:18}, priority = {2}, title = {Induction of pluripotent stem cells from adult human fibroblasts by defined factors.}, url = {http://dx.doi.org/10.1016/j.cell.2007.11.019}, volume = {131}, year = {2007} }

TY - JOUR ID - citeulike:2021374 L3 - citeulike-article-id:2021374 TI - Induction of pluripotent stem cells from adult human fibroblasts by defined factors. JF - Cell VL - 131 IS - 5 SP - 861 EP - 872 AD - Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan. SN - 0092-8674 N2 - Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts. KW - biomedical AU - Takahashi, K AU - Tanabe, K AU - Ohnuki, M AU - Narita, M AU - Ichisaka, T AU - Tomoda, K AU - Yamanaka, S PY - 2007/11/30/ UR - http://dx.doi.org/10.1016/j.cell.2007.11.019 ER -