Analysis of the vertebrate insulator protein CTCF-binding sites in the human genome.

@article{citeulike:1476474, abstract = {Insulator elements affect gene expression by preventing the spread of heterochromatin and restricting transcriptional enhancers from activation of unrelated promoters. In vertebrates, insulator's function requires association with the CCCTC-binding factor (CTCF), a protein that recognizes long and diverse nucleotide sequences. While insulators are critical in gene regulation, only a few have been reported. Here, we describe 13,804 CTCF-binding sites in potential insulators of the human genome, discovered experimentally in primary human fibroblasts. Most of these sequences are located far from the transcriptional start sites, with their distribution strongly correlated with genes. The majority of them fit to a consensus motif highly conserved and suitable for predicting possible insulators driven by CTCF in other vertebrate genomes. In addition, CTCF localization is largely invariant across different cell types. Our results provide a resource for investigating insulator function and possible other general and evolutionarily conserved activities of CTCF sites.}, address = {Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego School of Medicine, La Jolla, CA 92093-0653, USA. taehoon.kim@yale.edu}, author = {Kim, T. H. and Abdullaev, Z. K. and Smith, A. D. and Ching, K. A. and Loukinov, D. I. and Green, R. D. and Zhang, M. Q. and Lobanenkov, V. V. and Ren, B. }, citeulike-article-id = {1476474}, doi = {10.1016/j.cell.2006.12.048}, issn = {0092-8674}, journal = {Cell}, keywords = {ctcf, genomics, insulator}, month = {March}, number = {6}, pages = {1231--1245}, posted-at = {2008-05-15 17:33:00}, priority = {2}, title = {Analysis of the vertebrate insulator protein CTCF-binding sites in the human genome.}, url = {http://dx.doi.org/10.1016/j.cell.2006.12.048}, volume = {128}, year = {2007} }

TY - JOUR ID - citeulike:1476474 L3 - citeulike-article-id:1476474 TI - Analysis of the vertebrate insulator protein CTCF-binding sites in the human genome. JF - Cell VL - 128 IS - 6 SP - 1231 EP - 1245 AD - Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego School of Medicine, La Jolla, CA 92093-0653, USA. taehoon.kim@yale.edu SN - 0092-8674 N2 - Insulator elements affect gene expression by preventing the spread of heterochromatin and restricting transcriptional enhancers from activation of unrelated promoters. In vertebrates, insulator's function requires association with the CCCTC-binding factor (CTCF), a protein that recognizes long and diverse nucleotide sequences. While insulators are critical in gene regulation, only a few have been reported. Here, we describe 13,804 CTCF-binding sites in potential insulators of the human genome, discovered experimentally in primary human fibroblasts. Most of these sequences are located far from the transcriptional start sites, with their distribution strongly correlated with genes. The majority of them fit to a consensus motif highly conserved and suitable for predicting possible insulators driven by CTCF in other vertebrate genomes. In addition, CTCF localization is largely invariant across different cell types. Our results provide a resource for investigating insulator function and possible other general and evolutionarily conserved activities of CTCF sites. KW - ctcf KW - genomics KW - insulator AU - Kim, TH AU - Abdullaev, ZK AU - Smith, AD AU - Ching, KA AU - Loukinov, DI AU - Green, RD AU - Zhang, MQ AU - Lobanenkov, VV AU - Ren, B PY - 2007/03/23/ UR - http://dx.doi.org/10.1016/j.cell.2006.12.048 ER -