Serial analysis of gene expression.

by: VE Velculescu, L Zhang, B Vogelstein, KW Kinzler

Science, Vol. 270, No. 5235. (20 October 1995), pp. 484-487.

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Abstract

The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis of gene expression (SAGE), that allows the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1000 tags revealed a gene expression pattern characteristic of pancreatic function. New pancreatic transcripts corresponding to novel tags were identified. SAGE should provide a broadly applicable means for the quantitative cataloging and comparison of expressed genes in a variety of normal, developmental, and disease states.

@article{citeulike:770009, abstract = {The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis of gene expression (SAGE), that allows the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1000 tags revealed a gene expression pattern characteristic of pancreatic function. New pancreatic transcripts corresponding to novel tags were identified. SAGE should provide a broadly applicable means for the quantitative cataloging and comparison of expressed genes in a variety of normal, developmental, and disease states.}, address = {Oncology Center, Johns Hopkins University, Baltimore, MD 21231, USA.}, author = {Velculescu, V. E. and Zhang, L. and Vogelstein, B. and Kinzler, K. W. }, citeulike-article-id = {770009}, doi = {10.1126/science.270.5235.484}, issn = {0036-8075}, journal = {Science}, month = {October}, number = {5235}, pages = {484--487}, posted-at = {2008-05-13 11:15:30}, priority = {5}, title = {Serial analysis of gene expression.}, url = {http://dx.doi.org/10.1126/science.270.5235.484}, volume = {270}, year = {1995} }

RIS record

TY - JOUR ID - citeulike:770009 L3 - citeulike-article-id:770009 TI - Serial analysis of gene expression. JF - Science VL - 270 IS - 5235 SP - 484 EP - 487 AD - Oncology Center, Johns Hopkins University, Baltimore, MD 21231, USA. SN - 0036-8075 N2 - The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis of gene expression (SAGE), that allows the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1000 tags revealed a gene expression pattern characteristic of pancreatic function. New pancreatic transcripts corresponding to novel tags were identified. SAGE should provide a broadly applicable means for the quantitative cataloging and comparison of expressed genes in a variety of normal, developmental, and disease states. AU - Velculescu, VE AU - Zhang, L AU - Vogelstein, B AU - Kinzler, KW PY - 1995/10/20/ UR - http://dx.doi.org/10.1126/science.270.5235.484 ER -

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