The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles.

by: DT Miyamoto, ZE Perlman, KS Burbank, AC Groen, TJ Mitchison

J Cell Biol, Vol. 167, No. 5. (6 December 2004), pp. 813-818.

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Abstract

Although mitotic and meiotic spindles maintain a steady-state length during metaphase, their antiparallel microtubules slide toward spindle poles at a constant rate. This "poleward flux" of microtubules occurs in many organisms and may provide part of the force for chromosome segregation. We use quantitative image analysis to examine the role of the kinesin Eg5 in poleward flux in metaphase Xenopus laevis egg extract spindles. Pharmacological inhibition of Eg5 results in a dose-responsive slowing of flux, and biochemical depletion of Eg5 significantly decreases the flux rate. Our results suggest that ensembles of nonprocessive Eg5 motors drive flux in metaphase Xenopus extract spindles.

@article{citeulike:453895, abstract = {Although mitotic and meiotic spindles maintain a steady-state length during metaphase, their antiparallel microtubules slide toward spindle poles at a constant rate. This "poleward flux" of microtubules occurs in many organisms and may provide part of the force for chromosome segregation. We use quantitative image analysis to examine the role of the kinesin Eg5 in poleward flux in metaphase Xenopus laevis egg extract spindles. Pharmacological inhibition of Eg5 results in a dose-responsive slowing of flux, and biochemical depletion of Eg5 significantly decreases the flux rate. Our results suggest that ensembles of nonprocessive Eg5 motors drive flux in metaphase Xenopus extract spindles.}, address = {Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA. miyamoto@post.harvard.edu}, author = {Miyamoto, D. T. and Perlman, Z. E. and Burbank, K. S. and Groen, A. C. and Mitchison, T. J. }, citeulike-article-id = {453895}, doi = {10.1083/jcb.200407126}, issn = {0021-9525}, journal = {J Cell Biol}, keywords = {flux, measurement, speed}, month = {December}, number = {5}, pages = {813--818}, posted-at = {2005-12-31 21:33:37}, priority = {3}, title = {The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles.}, url = {http://dx.doi.org/10.1083/jcb.200407126}, volume = {167}, year = {2004} }

RIS record

TY - JOUR ID - citeulike:453895 L3 - citeulike-article-id:453895 TI - The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles. JF - J Cell Biol VL - 167 IS - 5 SP - 813 EP - 818 AD - Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA. miyamoto@post.harvard.edu SN - 0021-9525 N2 - Although mitotic and meiotic spindles maintain a steady-state length during metaphase, their antiparallel microtubules slide toward spindle poles at a constant rate. This "poleward flux" of microtubules occurs in many organisms and may provide part of the force for chromosome segregation. We use quantitative image analysis to examine the role of the kinesin Eg5 in poleward flux in metaphase Xenopus laevis egg extract spindles. Pharmacological inhibition of Eg5 results in a dose-responsive slowing of flux, and biochemical depletion of Eg5 significantly decreases the flux rate. Our results suggest that ensembles of nonprocessive Eg5 motors drive flux in metaphase Xenopus extract spindles. KW - flux KW - measurement KW - speed AU - Miyamoto, DT AU - Perlman, ZE AU - Burbank, KS AU - Groen, AC AU - Mitchison, TJ PY - 2004/12/06/ UR - http://dx.doi.org/10.1083/jcb.200407126 ER -

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