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TRACKING OF VIRUS PARTICLES IN TIME-LAPSE FLUORESCENCE MICROSCOPY IMAGE SEQUENCES

by: WJ Godinez, M Lampe, S Worz, B Muller, R Eils, K Rohr
Biomedical Imaging: From Nano to Macro, 2007. ISBI 2007. 4th IEEE International Symposium on (2007), pp. 256-259.


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Modern developments in time-lapse microscopy enable the observation of a variety of processes exhibited by viruses. The dynamic nature of these processes requires the tracking of viruses over time to explore the spatio-temporal relationships. In this work, we developed deterministic and probabilistic approaches for multiple virus tracking. A quantitative comparison based on synthetic image sequences was carried out to evaluate the performance of the different algorithms. We have also applied the algorithms to real microscopy images of HIV-1 particles and have compared the tracking results with ground truth obtained from manual tracking. It turns out that the probabilistic approach outperforms the deterministic schemes


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