Combination of cell-free expression and NMR spectroscopy as a new approach for structural investigation of membrane proteins.

@article{citeulike:887334, abstract = {Despite major technical advance in methods used for structural investigations of proteins structure determination of membrane proteins still poses a significant challenge. Recently, the application of cell-free expression systems to membrane proteins has demonstrated that this technique can be used to produce quantities sufficient for structural investigations for many different membrane proteins. In particular for NMR spectroscopy, cell-free expression provides major advantages since it allows for amino acid type selective and even amino acid position specific labeling. In this mini-review we discuss the combination of cell-free membrane protein expression and liquid state NMR spectroscopy.}, address = {Institute for Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Marie-Curie Str. 9, 60439 Frankfurt, Germany.}, author = {Koglin, A. and Klammt, C. and Trbovic, N. and Schwarz, D. and Schneider, B. and Sch\"{a}fer, B. and L\"{o}hr, F. and Bernhard, F. and D\"{o}tsch, V. }, citeulike-article-id = {887334}, doi = {http://dx.doi.org/10.1002/mrc.1833}, issn = {0749-1581}, journal = {Magn Reson Chem}, keywords = {cell\_free, membrane\_protein, nmr}, month = {July}, posted-at = {2008-07-24 08:50:26}, priority = {2}, title = {Combination of cell-free expression and NMR spectroscopy as a new approach for structural investigation of membrane proteins.}, url = {http://dx.doi.org/10.1002/mrc.1833}, volume = {44 Spec No}, year = {2006} }

TY - JOUR ID - citeulike:887334 L3 - citeulike-article-id:887334 TI - Combination of cell-free expression and NMR spectroscopy as a new approach for structural investigation of membrane proteins. JF - Magn Reson Chem VL - 44 Spec No AD - Institute for Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Marie-Curie Str. 9, 60439 Frankfurt, Germany. SN - 0749-1581 N2 - Despite major technical advance in methods used for structural investigations of proteins structure determination of membrane proteins still poses a significant challenge. Recently, the application of cell-free expression systems to membrane proteins has demonstrated that this technique can be used to produce quantities sufficient for structural investigations for many different membrane proteins. In particular for NMR spectroscopy, cell-free expression provides major advantages since it allows for amino acid type selective and even amino acid position specific labeling. In this mini-review we discuss the combination of cell-free membrane protein expression and liquid state NMR spectroscopy. KW - cell_free KW - membrane_protein KW - nmr AU - Koglin, A AU - Klammt, C AU - Trbovic, N AU - Schwarz, D AU - Schneider, B AU - Schäfer, B AU - Löhr, F AU - Bernhard, F AU - Dötsch, V PY - 2006/07// UR - http://dx.doi.org/10.1002/mrc.1833 ER -