The use of fura-2 for estimating ca buffers and ca fluxes

@article{citeulike:705766, abstract = {The compound fura-2 (Grynkiewicz et al., J. Biol. Chem. 260, 3440-3450, 1985) is generally known as an indicator dye for measuring the concentration of free calcium ([Ca2+]) inside living cells. It should be appreciated, however, that this is not what it actually is. More accurately, it is a divalent metal ion chelator which changes its fluorescence properties upon complexation. Thus, [Ca2+] has to be inferred indirectly by means of the law of mass action. As a chelator, fura-2 may influence the quantity of interest, the Ca signal. On the other hand, the chelator action may be used for a number of other purposes, some of them more directly related to its molecular properties: as a chelator, competing with endogenous Ca buffers, it can be used to estimate endogenous buffers and their properties. When present at sufficiently high concentration, such that it outcompetes endogenous buffers, fura-2 reports total Ca changes and is a probe for Ca fluxes across the membrane. Here, theory and methodological considerations of such applications of fura-2 will be summarized and results on Ca buffer and Ca flux measurements derived from various methods will be compared.}, author = {Neher, E. }, citeulike-article-id = {705766}, doi = {10.1016/0028-3908(95)00144-U}, journal = {Neuropharmacology}, keywords = {biophysics, buffer, calcium, flux, fura-2}, month = {November}, number = {11}, pages = {1423--1442}, posted-at = {2006-06-21 14:14:35}, priority = {3}, title = {The use of fura-2 for estimating ca buffers and ca fluxes}, url = {http://dx.doi.org/10.1016/0028-3908(95)00144-U}, volume = {34}, year = {1995} }

TY - JOUR ID - citeulike:705766 L3 - citeulike-article-id:705766 TI - The use of fura-2 for estimating ca buffers and ca fluxes JF - Neuropharmacology VL - 34 IS - 11 SP - 1423 EP - 1442 N2 - The compound fura-2 (Grynkiewicz et al., J. Biol. Chem. 260, 3440-3450, 1985) is generally known as an indicator dye for measuring the concentration of free calcium ([Ca2+]) inside living cells. It should be appreciated, however, that this is not what it actually is. More accurately, it is a divalent metal ion chelator which changes its fluorescence properties upon complexation. Thus, [Ca2+] has to be inferred indirectly by means of the law of mass action. As a chelator, fura-2 may influence the quantity of interest, the Ca signal. On the other hand, the chelator action may be used for a number of other purposes, some of them more directly related to its molecular properties: as a chelator, competing with endogenous Ca buffers, it can be used to estimate endogenous buffers and their properties. When present at sufficiently high concentration, such that it outcompetes endogenous buffers, fura-2 reports total Ca changes and is a probe for Ca fluxes across the membrane. Here, theory and methodological considerations of such applications of fura-2 will be summarized and results on Ca buffer and Ca flux measurements derived from various methods will be compared. KW - biophysics KW - buffer KW - calcium KW - flux KW - fura-2 AU - Neher, E PY - 1995/11// UR - http://dx.doi.org/10.1016/0028-3908(95)00144-U ER -