Cell shape provides global control of focal adhesion assembly

@article{citeulike:405494, abstract = {Cell spreading was controlled independently of the amount and density of immobilized integrin ligand by culturing cells on single adhesive islands of different sizes (100-2500 [mu]m2) and shapes (squares, circles, and lines) or on many smaller (3-5 [mu]m diameter) circular islands that were coated with a saturating density of fibronectin and separated by non-adhesive regions. The amount of focal adhesions (FAs) containing vinculin and phosphotyrosine increased in direct proportion to cell spreading under all conditions. FAs localized asymmetrically along the periphery of the small islands that experienced highest tensional stress, and FA staining increased when cytoskeletal tension was stimulated with thrombin, whereas inhibitors of contractility promoted FA disassembly. Thus, these findings demonstrate the existence of an "inside-out" mechanism whereby global cell distortion produces increases in cytoskeletal tension that feed back to drive local changes in FA assembly. This complex interplay between cell morphology, mechanics, and adhesion may be critical to how cells integrate from and function in living tissues.}, author = {Chen, Christopher S. and Alonso, Jose L. and Ostuni, Emanuele and Whitesides, George M. and Ingber, Donald E. }, citeulike-article-id = {405494}, doi = {10.1016/S0006-291X(03)01165-3}, journal = {Biochemical and Biophysical Research Communications}, keywords = {focal\_adhesion}, month = {July}, number = {2}, pages = {355--361}, posted-at = {2008-03-28 21:39:51}, priority = {2}, title = {Cell shape provides global control of focal adhesion assembly}, url = {http://dx.doi.org/10.1016/S0006-291X(03)01165-3}, volume = {307}, year = {2003} }

TY - JOUR ID - citeulike:405494 L3 - citeulike-article-id:405494 TI - Cell shape provides global control of focal adhesion assembly JF - Biochemical and Biophysical Research Communications VL - 307 IS - 2 SP - 355 EP - 361 N2 - Cell spreading was controlled independently of the amount and density of immobilized integrin ligand by culturing cells on single adhesive islands of different sizes (100-2500 [mu]m2) and shapes (squares, circles, and lines) or on many smaller (3-5 [mu]m diameter) circular islands that were coated with a saturating density of fibronectin and separated by non-adhesive regions. The amount of focal adhesions (FAs) containing vinculin and phosphotyrosine increased in direct proportion to cell spreading under all conditions. FAs localized asymmetrically along the periphery of the small islands that experienced highest tensional stress, and FA staining increased when cytoskeletal tension was stimulated with thrombin, whereas inhibitors of contractility promoted FA disassembly. Thus, these findings demonstrate the existence of an "inside-out" mechanism whereby global cell distortion produces increases in cytoskeletal tension that feed back to drive local changes in FA assembly. This complex interplay between cell morphology, mechanics, and adhesion may be critical to how cells integrate from and function in living tissues. KW - focal_adhesion AU - Chen, Christopher AU - Alonso, Jose AU - Ostuni, Emanuele AU - Whitesides, George AU - Ingber, Donald PY - 2003/07/25/ UR - http://dx.doi.org/10.1016/S0006-291X(03)01165-3 ER -