Structure-based assembly of protein complexes in yeast.

@article{aloy2004, abstract = {Images of entire cells are preceding atomic structures of the separate molecular machines that they contain. The resulting gap in knowledge can be partly bridged by protein-protein interactions, bioinformatics, and electron microscopy. Here we use interactions of known three-dimensional structure to model a large set of yeast complexes, which we also screen by electron microscopy. For 54 of 102 complexes, we obtain at least partial models of interacting subunits. For 29, including the exosome, the chaperonin containing TCP-1, a 3'-messenger RNA degradation complex, and RNA polymerase II, the process suggests atomic details not easily seen by homology, involving the combination of two or more known structures. We also consider interactions between complexes (cross-talk) and use these to construct a structure-based network of molecular machines in the cell.}, address = {European Molecular Biology Laboratory, Structural and Computational Biology Programme, 1, 69117 Heidelberg, Germany.}, author = {Aloy, P. and Boettcher, B. and Ceulemans, H. and Leutwein, C. and Mellwig, C. and Fischer, S. and Gavin, A. C. and Bork, P. and Superti-Furga, G. and Serrano, L. and Russell, R. B. }, citeulike-article-id = {708179}, doi = {10.1126/science.1092645}, issn = {1095-9203}, journal = {Science}, keywords = {chapter, ppi}, month = {March}, number = {5666}, pages = {2026--2029}, posted-at = {2008-03-20 11:42:58}, priority = {2}, title = {Structure-based assembly of protein complexes in yeast.}, url = {http://dx.doi.org/10.1126/science.1092645}, volume = {303}, year = {2004} }

TY - JOUR ID - aloy2004 L3 - citeulike-article-id:708179 TI - Structure-based assembly of protein complexes in yeast. JF - Science VL - 303 IS - 5666 SP - 2026 EP - 2029 AD - European Molecular Biology Laboratory, Structural and Computational Biology Programme, 1, 69117 Heidelberg, Germany. SN - 1095-9203 N2 - Images of entire cells are preceding atomic structures of the separate molecular machines that they contain. The resulting gap in knowledge can be partly bridged by protein-protein interactions, bioinformatics, and electron microscopy. Here we use interactions of known three-dimensional structure to model a large set of yeast complexes, which we also screen by electron microscopy. For 54 of 102 complexes, we obtain at least partial models of interacting subunits. For 29, including the exosome, the chaperonin containing TCP-1, a 3'-messenger RNA degradation complex, and RNA polymerase II, the process suggests atomic details not easily seen by homology, involving the combination of two or more known structures. We also consider interactions between complexes (cross-talk) and use these to construct a structure-based network of molecular machines in the cell. KW - chapter KW - ppi AU - Aloy, P AU - Boettcher, B AU - Ceulemans, H AU - Leutwein, C AU - Mellwig, C AU - Fischer, S AU - Gavin, AC AU - Bork, P AU - Superti-Furga, G AU - Serrano, L AU - Russell, RB PY - 2004/03/26/ UR - http://dx.doi.org/10.1126/science.1092645 ER -