Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi.

@article{citeulike:2096382, abstract = {Microsporidia are obligate intracellular parasites infesting many animal groups. Lacking mitochondria and peroxysomes, these unicellular eukaryotes were first considered a deeply branching protist lineage that diverged before the endosymbiotic event that led to mitochondria. The discovery of a gene for a mitochondrial-type chaperone combined with molecular phylogenetic data later implied that microsporidia are atypical fungi that lost mitochondria during evolution. Here we report the DNA sequences of the 11 chromosomes of the approximately 2.9-megabase (Mb) genome of Encephalitozoon cuniculi (1,997 potential protein-coding genes). Genome compaction is reflected by reduced intergenic spacers and by the shortness of most putative proteins relative to their eukaryote orthologues. The strong host dependence is illustrated by the lack of genes for some biosynthetic pathways and for the tricarboxylic acid cycle. Phylogenetic analysis lends substantial credit to the fungal affiliation of microsporidia. Because the E. cuniculi genome contains genes related to some mitochondrial functions (for example, Fe-S cluster assembly), we hypothesize that microsporidia have retained a mitochondrion-derived organelle.}, address = {Genoscope, UMR CNRS 8030, CP 5706, 91057 Evry cedex, France.}, author = {Katinka, M. D. and Duprat, S. and Cornillot, E. and M\'{e}t\'{e}nier, G. and Thomarat, F. and Prensier, G. and Barbe, V. and Peyretaillade, E. and Brottier, P. and Wincker, P. and Delbac, F. and El Alaoui, H. and Peyret, P. and Saurin, W. and Gouy, M. and Weissenbach, J. and Vivar\`{e}s, C. P. }, citeulike-article-id = {2096382}, doi = {10.1038/35106579}, issn = {0028-0836}, journal = {Nature}, keywords = {evolution, genomics, methodspaper, mitochondria, parts-list, prediction}, month = {November}, number = {6862}, pages = {450--453}, posted-at = {2008-04-25 15:19:22}, priority = {2}, title = {Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi.}, url = {http://dx.doi.org/10.1038/35106579}, volume = {414}, year = {2001} }

TY - JOUR ID - citeulike:2096382 L3 - citeulike-article-id:2096382 TI - Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi. JF - Nature VL - 414 IS - 6862 SP - 450 EP - 453 AD - Genoscope, UMR CNRS 8030, CP 5706, 91057 Evry cedex, France. SN - 0028-0836 N2 - Microsporidia are obligate intracellular parasites infesting many animal groups. Lacking mitochondria and peroxysomes, these unicellular eukaryotes were first considered a deeply branching protist lineage that diverged before the endosymbiotic event that led to mitochondria. The discovery of a gene for a mitochondrial-type chaperone combined with molecular phylogenetic data later implied that microsporidia are atypical fungi that lost mitochondria during evolution. Here we report the DNA sequences of the 11 chromosomes of the approximately 2.9-megabase (Mb) genome of Encephalitozoon cuniculi (1,997 potential protein-coding genes). Genome compaction is reflected by reduced intergenic spacers and by the shortness of most putative proteins relative to their eukaryote orthologues. The strong host dependence is illustrated by the lack of genes for some biosynthetic pathways and for the tricarboxylic acid cycle. Phylogenetic analysis lends substantial credit to the fungal affiliation of microsporidia. Because the E. cuniculi genome contains genes related to some mitochondrial functions (for example, Fe-S cluster assembly), we hypothesize that microsporidia have retained a mitochondrion-derived organelle. KW - evolution KW - genomics KW - methodspaper KW - mitochondria KW - parts-list KW - prediction AU - Katinka, MD AU - Duprat, S AU - Cornillot, E AU - Méténier, G AU - Thomarat, F AU - Prensier, G AU - Barbe, V AU - Peyretaillade, E AU - Brottier, P AU - Wincker, P AU - Delbac, F AU - El Alaoui, H AU - Peyret, P AU - Saurin, W AU - Gouy, M AU - Weissenbach, J AU - Vivarès, CP PY - 2001/11/22/ UR - http://dx.doi.org/10.1038/35106579 ER -