Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789

@article{citeulike:1530884, abstract = {We sequenced the genome of Saccharomyces cerevisiae strain YJM789, which was derived from a yeast isolated from the lung of an AIDS patient with pneumonia. The strain is used for studies of fungal infections and quantitative genetics because of its extensive phenotypic differences to the laboratory reference strain, including growth at high temperature and deadly virulence in mouse models. Here we show that the {approx}12-Mb genome of YJM789 contains {approx}60,000 SNPs and {approx}6,000 indels with respect to the reference S288c genome, leading to protein polymorphisms with a few known cases of phenotypic changes. Several ORFs are found to be unique to YJM789, some of which might have been acquired through horizontal transfer. Localized regions of high polymorphism density are scattered over the genome, in some cases spanning multiple ORFs and in others concentrated within single genes. The sequence of YJM789 contains clues to pathogenicity and spurs the development of more powerful approaches to dissecting the genetic basis of complex hereditary traits. 10.1073/pnas.0701291104}, author = {Wei, Wu and Mccusker, John H. and Hyman, Richard W. and Jones, Ted and Ning, Ye and Cao, Zhiwei and Gu, Zhenglong and Bruno, Dan and Miranda, Molly and Nguyen, Michelle and Wilhelmy, Julie and Komp, Caridad and Tamse, Raquel and Wang, Xiaojing and Jia, Peilin and Luedi, Philippe and Oefner, Peter J. and David, Lior and Dietrich, Fred S. and Li, Yixue and Davis, Ronald W. and Steinmetz, Lars M. }, citeulike-article-id = {1530884}, doi = {http://dx.doi.org/10.1073/pnas.0701291104}, journal = {PNAS}, keywords = {bioinformatics, genomics, yeast, yjm789}, month = {July}, number = {31}, pages = {12825--12830}, posted-at = {2007-08-02 16:15:02}, priority = {0}, title = {Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789}, url = {http://dx.doi.org/10.1073/pnas.0701291104}, volume = {104}, year = {2007} }

TY - JOUR ID - citeulike:1530884 L3 - citeulike-article-id:1530884 TI - Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789 JF - PNAS VL - 104 IS - 31 SP - 12825 EP - 12830 N2 - We sequenced the genome of Saccharomyces cerevisiae strain YJM789, which was derived from a yeast isolated from the lung of an AIDS patient with pneumonia. The strain is used for studies of fungal infections and quantitative genetics because of its extensive phenotypic differences to the laboratory reference strain, including growth at high temperature and deadly virulence in mouse models. Here we show that the {approx}12-Mb genome of YJM789 contains {approx}60,000 SNPs and {approx}6,000 indels with respect to the reference S288c genome, leading to protein polymorphisms with a few known cases of phenotypic changes. Several ORFs are found to be unique to YJM789, some of which might have been acquired through horizontal transfer. Localized regions of high polymorphism density are scattered over the genome, in some cases spanning multiple ORFs and in others concentrated within single genes. The sequence of YJM789 contains clues to pathogenicity and spurs the development of more powerful approaches to dissecting the genetic basis of complex hereditary traits. 10.1073/pnas.0701291104 KW - bioinformatics KW - genomics KW - yeast KW - yjm789 AU - Wei, Wu AU - Mccusker, John AU - Hyman, Richard AU - Jones, Ted AU - Ning, Ye AU - Cao, Zhiwei AU - Gu, Zhenglong AU - Bruno, Dan AU - Miranda, Molly AU - Nguyen, Michelle AU - Wilhelmy, Julie AU - Komp, Caridad AU - Tamse, Raquel AU - Wang, Xiaojing AU - Jia, Peilin AU - Luedi, Philippe AU - Oefner, Peter AU - David, Lior AU - Dietrich, Fred AU - Li, Yixue AU - Davis, Ronald AU - Steinmetz, Lars PY - 2007/07/31/ UR - http://dx.doi.org/10.1073/pnas.0701291104 ER -