Sizing the Bacillus anthracis PA63 Channel with Nonelectrolyte Poly(Ethylene Glycols)

by: Brian J Nablo, Kelly M Halverson, Joseph W Robertson, Tam L Nguyen, Rekha G Panchal, Rick Gussio, Sina Bavari, Oleg V Krasilnikov, John J Kasianowicz

Biophys. J., Vol. 95, No. 3. (1 August 2008), pp. 1157-1164.

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Abstract

Nonelectrolyte polymers of poly(ethylene glycol) (PEG) were used to estimate the diameter of the ion channel formed by the Bacillus anthracis protective antigen 63 (PA63). Based on the ability of different molecular weight PEGs to partition into the pore and reduce channel conductance, the pore appears to be narrower than the one formed by Staphylococcus aureus alpha-hemolysin. Numerical integration of the PEG sample mass spectra and the channel conductance data were used to refine the estimate of the pore's PEG molecular mass cutoff ([~]1400 g/mol). The results suggest that the limiting diameter of the PA63 pore is <2 nm, which is consistent with an all-atom model of the PA63 channel and previous experiments using large ions. 10.1529/biophysj.107.121715

@article{citeulike:3035519, abstract = {Nonelectrolyte polymers of poly(ethylene glycol) (PEG) were used to estimate the diameter of the ion channel formed by the Bacillus anthracis protective antigen 63 (PA63). Based on the ability of different molecular weight PEGs to partition into the pore and reduce channel conductance, the pore appears to be narrower than the one formed by Staphylococcus aureus {alpha}-hemolysin. Numerical integration of the PEG sample mass spectra and the channel conductance data were used to refine the estimate of the pore's PEG molecular mass cutoff ([~]1400 g/mol). The results suggest that the limiting diameter of the PA63 pore is <2 nm, which is consistent with an all-atom model of the PA63 channel and previous experiments using large ions. 10.1529/biophysj.107.121715}, author = {Nablo, Brian J. and Halverson, Kelly M. and Robertson, Joseph W. and Nguyen, Tam L. and Panchal, Rekha G. and Gussio, Rick and Bavari, Sina and Krasilnikov, Oleg V. and Kasianowicz, John J. }, citeulike-article-id = {3035519}, doi = {10.1529/biophysj.107.121715}, journal = {Biophys. J.}, keywords = {block, channel, peg, pore, size}, month = {August}, number = {3}, pages = {1157--1164}, posted-at = {2008-07-23 01:09:22}, priority = {2}, title = {Sizing the Bacillus anthracis PA63 Channel with Nonelectrolyte Poly(Ethylene Glycols)}, url = {http://dx.doi.org/10.1529/biophysj.107.121715}, volume = {95}, year = {2008} }

RIS record

TY - JOUR ID - citeulike:3035519 L3 - citeulike-article-id:3035519 TI - Sizing the Bacillus anthracis PA63 Channel with Nonelectrolyte Poly(Ethylene Glycols) JF - Biophys. J. VL - 95 IS - 3 SP - 1157 EP - 1164 N2 - Nonelectrolyte polymers of poly(ethylene glycol) (PEG) were used to estimate the diameter of the ion channel formed by the Bacillus anthracis protective antigen 63 (PA63). Based on the ability of different molecular weight PEGs to partition into the pore and reduce channel conductance, the pore appears to be narrower than the one formed by Staphylococcus aureus {alpha}-hemolysin. Numerical integration of the PEG sample mass spectra and the channel conductance data were used to refine the estimate of the pore's PEG molecular mass cutoff ([~]1400 g/mol). The results suggest that the limiting diameter of the PA63 pore is <2 nm, which is consistent with an all-atom model of the PA63 channel and previous experiments using large ions. 10.1529/biophysj.107.121715 KW - block KW - channel KW - peg KW - pore KW - size AU - Nablo, Brian AU - Halverson, Kelly AU - Robertson, Joseph AU - Nguyen, Tam AU - Panchal, Rekha AU - Gussio, Rick AU - Bavari, Sina AU - Krasilnikov, Oleg AU - Kasianowicz, John PY - 2008/08/01/ UR - http://dx.doi.org/10.1529/biophysj.107.121715 ER -

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