Reduction of PTP1B by RNAi upregulates the activity of insulin controlled fatty acid synthase promoter.

@article{citeulike:228555, abstract = {Metabolic deregulation accompanying type II diabetes is characterized by insulin resistance in peripheral tissues (liver, muscle, and adipose), mediated by impairments in insulin receptor (IR) signaling. Protein tyrosine phosphatase 1B (PTP1B) has been shown to be a negative regulator of IR autophosphorylation and thus has been considered as a major therapeutic target for the treatment of type II diabetes. We use RNA interference technique to downregulate PTP1B expression in hepatoma cell line. A secretory HBV s-antigen was introduced as reporter and driven by mouse fatty acid synthase promoter, which is positively controlled by insulin signaling. Liver-targeted hydrodynamic injection in tail vein was introduced to transfer siRNA (or siRNA expression vector) and reporter plasmid into mouse liver. On fasted/refed and glucose stimulation condition, the HBV s-antigen in sera in RNAi group was higher than that in the negative group. Our results provided evidence that upregulation of insulin signaling by reducing PTP1B liver with RNAi can be a potent diabetes treatment method.}, address = {Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai 200433, China.}, author = {Xu, J. and Li, L. and Qian, Z. and Hong, J. and Shen, S. and Huang, W. }, citeulike-article-id = {228555}, doi = {http://dx.doi.org/10.1016/j.bbrc.2005.02.016}, issn = {0006-291X}, journal = {Biochem Biophys Res Commun}, keywords = {injection, irna, tail, vein}, month = {April}, number = {2}, pages = {538--543}, posted-at = {2005-06-15 17:25:14}, priority = {3}, title = {Reduction of PTP1B by RNAi upregulates the activity of insulin controlled fatty acid synthase promoter.}, url = {http://dx.doi.org/10.1016/j.bbrc.2005.02.016}, volume = {329}, year = {2005} }

TY - JOUR ID - citeulike:228555 L3 - citeulike-article-id:228555 TI - Reduction of PTP1B by RNAi upregulates the activity of insulin controlled fatty acid synthase promoter. JF - Biochem Biophys Res Commun VL - 329 IS - 2 SP - 538 EP - 543 AD - Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai 200433, China. SN - 0006-291X N2 - Metabolic deregulation accompanying type II diabetes is characterized by insulin resistance in peripheral tissues (liver, muscle, and adipose), mediated by impairments in insulin receptor (IR) signaling. Protein tyrosine phosphatase 1B (PTP1B) has been shown to be a negative regulator of IR autophosphorylation and thus has been considered as a major therapeutic target for the treatment of type II diabetes. We use RNA interference technique to downregulate PTP1B expression in hepatoma cell line. A secretory HBV s-antigen was introduced as reporter and driven by mouse fatty acid synthase promoter, which is positively controlled by insulin signaling. Liver-targeted hydrodynamic injection in tail vein was introduced to transfer siRNA (or siRNA expression vector) and reporter plasmid into mouse liver. On fasted/refed and glucose stimulation condition, the HBV s-antigen in sera in RNAi group was higher than that in the negative group. Our results provided evidence that upregulation of insulin signaling by reducing PTP1B liver with RNAi can be a potent diabetes treatment method. KW - injection KW - irna KW - tail KW - vein AU - Xu, J AU - Li, L AU - Qian, Z AU - Hong, J AU - Shen, S AU - Huang, W PY - 2005/04/08/ UR - http://dx.doi.org/10.1016/j.bbrc.2005.02.016 ER -