Identification of the HIV-1 gp41 core-binding motif - HXXNPF

@article{citeulike:2370021, abstract = {The HIV-1 gp41 core, a six-helix bundle formed between the N- and C-terminal heptad repeats, plays a critical role in fusion between the viral and target cell membranes. Using N36(L8)C34 as a model of the gp41 core to screen phage display peptide libraries, we identified a common motif, HXXNPF (X is any of the 20 natural amino acid residues). A selected positive phage clone L7.8 specifically bound to N36(L8)C34 and this binding could be blocked by a gp41 core-specific monoclonal antibody (NC-1). JCH-4, a peptide containing HXXNPF motif, effectively inhibited HIV-1 envelope glycoprotein-mediated syncytium-formation. The epitope of JCH-4 was proven to be linear and might locate in the NHR regions of the gp41 core. These data suggest that HXXNPF motif may be a gp41 core-binding sequence and HXXNPF motif-containing molecules can be used as probes for studying the role of the HIV-1 gp41 core in membrane fusion process.}, author = {Huang, Jing-He and Liu, Zu-Qiang and Liu, Shuwen and Jiang, Shibo and Chen, Ying-Hua }, citeulike-article-id = {2370021}, doi = {http://dx.doi.org/10.1016/j.febslet.2006.07.067}, journal = {FEBS Letters}, keywords = {antibodies, antibody, interactions, motif, protein-interaction, protein-protein}, month = {September}, number = {20}, pages = {4807--4814}, posted-at = {2008-02-13 15:02:26}, priority = {3}, title = {Identification of the HIV-1 gp41 core-binding motif - HXXNPF}, url = {http://dx.doi.org/10.1016/j.febslet.2006.07.067}, volume = {580}, year = {2006} }

TY - JOUR ID - citeulike:2370021 L3 - citeulike-article-id:2370021 TI - Identification of the HIV-1 gp41 core-binding motif - HXXNPF JF - FEBS Letters VL - 580 IS - 20 SP - 4807 EP - 4814 N2 - The HIV-1 gp41 core, a six-helix bundle formed between the N- and C-terminal heptad repeats, plays a critical role in fusion between the viral and target cell membranes. Using N36(L8)C34 as a model of the gp41 core to screen phage display peptide libraries, we identified a common motif, HXXNPF (X is any of the 20 natural amino acid residues). A selected positive phage clone L7.8 specifically bound to N36(L8)C34 and this binding could be blocked by a gp41 core-specific monoclonal antibody (NC-1). JCH-4, a peptide containing HXXNPF motif, effectively inhibited HIV-1 envelope glycoprotein-mediated syncytium-formation. The epitope of JCH-4 was proven to be linear and might locate in the NHR regions of the gp41 core. These data suggest that HXXNPF motif may be a gp41 core-binding sequence and HXXNPF motif-containing molecules can be used as probes for studying the role of the HIV-1 gp41 core in membrane fusion process. KW - antibodies KW - antibody KW - interactions KW - motif KW - protein-interaction KW - protein-protein AU - Huang, Jing-He AU - Liu, Zu-Qiang AU - Liu, Shuwen AU - Jiang, Shibo AU - Chen, Ying-Hua PY - 2006/09/04/ UR - http://dx.doi.org/10.1016/j.febslet.2006.07.067 ER -