Terminal proteins of Streptomyces chromosome can target DNA into eukaryotic nuclei

@article{citeulike:2800594, abstract = {Streptomyces species are highly abundant soil bacteria that possess linear chromosomes (and linear plasmids). The 5' ends of these molecules are covalently bound by terminal proteins (TPs), that are important for integrity and replication of the telomeres. There are at least two types of TPs, both of which contain a DNA-binding domain and a classical eukaryotic nuclear localization signal (NLS). Here we show that the NLS motifs on these TPs are highly efficient in targeting the proteins along with covalently bound plasmid DNA into the nuclei of human cells. The TP-mediated nuclear targeting resembles the inter-kingdom gene transfer mediated by Ti plasmids of Agrobacterium tumefaciens, in which a piece of the Ti plasmid DNA is targeted to the plant nuclei by a covalently bound NLS-containing protein. The discovery of the nuclear localization functions of the Streptomyces TPs not only suggests possible inter-kingdom gene exchanges between Streptomyces and eukaryotes in soil but also provides a novel strategy for gene delivery in humans and other eukaryotes. 10.1093/nar/gkm1170}, author = {Tsai, Hsiu-Hui and Huang, Chih-Hung and Lin, Alan M. and Chen, Carton W. }, citeulike-article-id = {2800594}, doi = {10.1093/nar/gkm1170}, journal = {Nucl. Acids Res.}, keywords = {eukaryotic, nucleus, protein, scl, streptomyces, targeting}, month = {May}, pages = {gkm1170+}, posted-at = {2008-05-15 04:29:13}, priority = {2}, title = {Terminal proteins of Streptomyces chromosome can target DNA into eukaryotic nuclei}, url = {http://dx.doi.org/10.1093/nar/gkm1170}, year = {2008} }

TY - JOUR ID - citeulike:2800594 L3 - citeulike-article-id:2800594 TI - Terminal proteins of Streptomyces chromosome can target DNA into eukaryotic nuclei JF - Nucl. Acids Res. SP - gkm1170 N2 - Streptomyces species are highly abundant soil bacteria that possess linear chromosomes (and linear plasmids). The 5' ends of these molecules are covalently bound by terminal proteins (TPs), that are important for integrity and replication of the telomeres. There are at least two types of TPs, both of which contain a DNA-binding domain and a classical eukaryotic nuclear localization signal (NLS). Here we show that the NLS motifs on these TPs are highly efficient in targeting the proteins along with covalently bound plasmid DNA into the nuclei of human cells. The TP-mediated nuclear targeting resembles the inter-kingdom gene transfer mediated by Ti plasmids of Agrobacterium tumefaciens, in which a piece of the Ti plasmid DNA is targeted to the plant nuclei by a covalently bound NLS-containing protein. The discovery of the nuclear localization functions of the Streptomyces TPs not only suggests possible inter-kingdom gene exchanges between Streptomyces and eukaryotes in soil but also provides a novel strategy for gene delivery in humans and other eukaryotes. 10.1093/nar/gkm1170 KW - eukaryotic KW - nucleus KW - protein KW - scl KW - streptomyces KW - targeting AU - Tsai, Hsiu-Hui AU - Huang, Chih-Hung AU - Lin, Alan AU - Chen, Carton PY - 2008/05/14/ UR - http://dx.doi.org/10.1093/nar/gkm1170 ER -