Gene expression profiling in postmortem Rett Syndrome brain: differential gene expression and patient classification.

@article{citeulike:1140419, abstract = {The identification of mutations in the transcriptional repressor methyl-CpG-binding protein 2 (MECP2) gene in Rett Syndrome (RTT) suggests that an inappropriate release of transcriptional silencing may give rise to RTT neuropathology. Despite this progress, the molecular basis of RTT neuropathogenesis remains unclear. Using multiple cDNA microarray technologies, subtractive hybridization, and conventional biochemistry, we generated comprehensive gene expression profiles of postmortem brain tissue from RTT patients and matched controls. Many glial transcripts involved in known neuropathological mechanisms were found to have increased expression in RTT brain, while decreases were observed in the expression of multiple neuron-specific mRNAs. Dramatic and consistent decreases in transcripts encoding presynaptic markers indicated a specific deficit in presynaptic development. Employing multiple clustering algorithms, it was possible to accurately segregate RTT from control brain tissue samples based solely on gene expression profile. Although previously achieved in cancers, our results constitute the first report of human disease classification using gene expression profiling in a complex tissue source such as brain.}, address = {Department of Neurology, Kennedy Krieger Institute, 707 North Broadway, Maryland, Baltimore 21205, USA.}, author = {Colantuoni, C. and Jeon, O. H. and Hyder, K. and Chenchik, A. and Khimani, A. H. and Narayanan, V. and Hoffman, E. P. and Kaufmann, W. E. and Naidu, S. and Pevsner, J. }, citeulike-article-id = {1140419}, doi = {http://dx.doi.org/10.1006/nbdi.2001.0428}, issn = {0969-9961}, journal = {Neurobiol Dis}, keywords = {and, brain, classification, differential, expression, gene, patient, profiling}, month = {October}, number = {5}, pages = {847--865}, posted-at = {2007-03-05 03:31:02}, priority = {2}, title = {Gene expression profiling in postmortem Rett Syndrome brain: differential gene expression and patient classification.}, url = {http://dx.doi.org/10.1006/nbdi.2001.0428}, volume = {8}, year = {2001} }

TY - JOUR ID - citeulike:1140419 L3 - citeulike-article-id:1140419 TI - Gene expression profiling in postmortem Rett Syndrome brain: differential gene expression and patient classification. JF - Neurobiol Dis VL - 8 IS - 5 SP - 847 EP - 865 AD - Department of Neurology, Kennedy Krieger Institute, 707 North Broadway, Maryland, Baltimore 21205, USA. SN - 0969-9961 N2 - The identification of mutations in the transcriptional repressor methyl-CpG-binding protein 2 (MECP2) gene in Rett Syndrome (RTT) suggests that an inappropriate release of transcriptional silencing may give rise to RTT neuropathology. Despite this progress, the molecular basis of RTT neuropathogenesis remains unclear. Using multiple cDNA microarray technologies, subtractive hybridization, and conventional biochemistry, we generated comprehensive gene expression profiles of postmortem brain tissue from RTT patients and matched controls. Many glial transcripts involved in known neuropathological mechanisms were found to have increased expression in RTT brain, while decreases were observed in the expression of multiple neuron-specific mRNAs. Dramatic and consistent decreases in transcripts encoding presynaptic markers indicated a specific deficit in presynaptic development. Employing multiple clustering algorithms, it was possible to accurately segregate RTT from control brain tissue samples based solely on gene expression profile. Although previously achieved in cancers, our results constitute the first report of human disease classification using gene expression profiling in a complex tissue source such as brain. KW - and KW - brain KW - classification KW - differential KW - expression KW - gene KW - patient KW - profiling AU - Colantuoni, C AU - Jeon, OH AU - Hyder, K AU - Chenchik, A AU - Khimani, AH AU - Narayanan, V AU - Hoffman, EP AU - Kaufmann, WE AU - Naidu, S AU - Pevsner, J PY - 2001/10// UR - http://dx.doi.org/10.1006/nbdi.2001.0428 ER -