Functional Architecture and Evolution of Transcriptional Elements That Drive Gene Coexpression

by: Christopher D Brown, David S Johnson, Arend Sidow

Science, Vol. 317, No. 5844. (14 September 2007), pp. 1557-1560.

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Abstract

Transcriptional coexpression of interacting gene products is required for complex molecular processes; however, the function and evolution of cis-regulatory elements that orchestrate coexpression remain largely unexplored. We mutagenized 19 regulatory elements that drive coexpression of Ciona muscle genes and obtained quantitative estimates of the cis-regulatory activity of the 77 motifs that comprise these elements. We found that individual motif activity ranges broadly within and among elements, and among different instantiations of the same motif type. The activity of orthologous motifs is strongly constrained, although motif arrangement, type, and activity vary greatly among the elements of different co-regulated genes. Thus, the syntactical rules governing this regulatory function are flexible but become highly constrained evolutionarily once they are established in a particular element. 10.1126/science.1145893

@article{citeulike:1658248, abstract = {Transcriptional coexpression of interacting gene products is required for complex molecular processes; however, the function and evolution of cis-regulatory elements that orchestrate coexpression remain largely unexplored. We mutagenized 19 regulatory elements that drive coexpression of Ciona muscle genes and obtained quantitative estimates of the cis-regulatory activity of the 77 motifs that comprise these elements. We found that individual motif activity ranges broadly within and among elements, and among different instantiations of the same motif type. The activity of orthologous motifs is strongly constrained, although motif arrangement, type, and activity vary greatly among the elements of different co-regulated genes. Thus, the syntactical rules governing this regulatory function are flexible but become highly constrained evolutionarily once they are established in a particular element. 10.1126/science.1145893}, author = {Brown, Christopher D. and Johnson, David S. and Sidow, Arend }, citeulike-article-id = {1658248}, doi = {10.1126/science.1145893}, journal = {Science}, keywords = {ciona, cis-trans, cluster, cns-differences, combination, enhanceosome, pwm}, month = {September}, number = {5844}, pages = {1557--1560}, posted-at = {2008-02-29 17:27:57}, priority = {2}, title = {Functional Architecture and Evolution of Transcriptional Elements That Drive Gene Coexpression}, url = {http://dx.doi.org/10.1126/science.1145893}, volume = {317}, year = {2007} }

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TY - JOUR ID - citeulike:1658248 L3 - citeulike-article-id:1658248 TI - Functional Architecture and Evolution of Transcriptional Elements That Drive Gene Coexpression JF - Science VL - 317 IS - 5844 SP - 1557 EP - 1560 N2 - Transcriptional coexpression of interacting gene products is required for complex molecular processes; however, the function and evolution of cis-regulatory elements that orchestrate coexpression remain largely unexplored. We mutagenized 19 regulatory elements that drive coexpression of Ciona muscle genes and obtained quantitative estimates of the cis-regulatory activity of the 77 motifs that comprise these elements. We found that individual motif activity ranges broadly within and among elements, and among different instantiations of the same motif type. The activity of orthologous motifs is strongly constrained, although motif arrangement, type, and activity vary greatly among the elements of different co-regulated genes. Thus, the syntactical rules governing this regulatory function are flexible but become highly constrained evolutionarily once they are established in a particular element. 10.1126/science.1145893 KW - ciona KW - cis-trans KW - cluster KW - cns-differences KW - combination KW - enhanceosome KW - pwm AU - Brown, Christopher AU - Johnson, David AU - Sidow, Arend PY - 2007/09/14/ UR - http://dx.doi.org/10.1126/science.1145893 ER -

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