Self-Assembled Water-Soluble Nucleic Acid Probe Tiles for Label-Free RNA Hybridization Assays

by: Yonggang Ke, Stuart Lindsay, Yung Chang, Yan Liu, Hao Yan

Science, Vol. 319, No. 5860. (11 January 2008), pp. 180-183.

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Abstract

The DNA origami method, in which long, single-stranded DNA segments are folded into shapes by short staple segments, was used to create nucleic acid probe tiles that are molecular analogs of macroscopic DNA chips. One hundred trillion probe tiles were fabricated in one step and bear pairs of 20-nucleotide-long single-stranded DNA segments that act as probe sequences. These tiles can hybridize to their targets in solution and, after adsorption onto mica surfaces, can be examined by atomic force microscopy in order to quantify binding events, because the probe segments greatly increase in stiffness upon hybridization. The nucleic acid probe tiles have been used to study position-dependent hybridization on the nanoscale and have also been used for label-free detection of RNA. 10.1126/science.1150082

@article{citeulike:2216617, abstract = {The DNA origami method, in which long, single-stranded DNA segments are folded into shapes by short staple segments, was used to create nucleic acid probe tiles that are molecular analogs of macroscopic DNA chips. One hundred trillion probe tiles were fabricated in one step and bear pairs of 20-nucleotide-long single-stranded DNA segments that act as probe sequences. These tiles can hybridize to their targets in solution and, after adsorption onto mica surfaces, can be examined by atomic force microscopy in order to quantify binding events, because the probe segments greatly increase in stiffness upon hybridization. The nucleic acid probe tiles have been used to study position-dependent hybridization on the nanoscale and have also been used for label-free detection of RNA. 10.1126/science.1150082}, author = {Ke, Yonggang and Lindsay, Stuart and Chang, Yung and Liu, Yan and Yan, Hao }, citeulike-article-id = {2216617}, doi = {http://dx.doi.org/10.1126/science.1150082}, journal = {Science}, keywords = {h, method}, month = {January}, number = {5860}, pages = {180--183}, posted-at = {2008-01-10 23:22:19}, priority = {2}, title = {Self-Assembled Water-Soluble Nucleic Acid Probe Tiles for Label-Free RNA Hybridization Assays}, url = {http://dx.doi.org/10.1126/science.1150082}, volume = {319}, year = {2008} }

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TY - JOUR ID - citeulike:2216617 L3 - citeulike-article-id:2216617 TI - Self-Assembled Water-Soluble Nucleic Acid Probe Tiles for Label-Free RNA Hybridization Assays JF - Science VL - 319 IS - 5860 SP - 180 EP - 183 N2 - The DNA origami method, in which long, single-stranded DNA segments are folded into shapes by short staple segments, was used to create nucleic acid probe tiles that are molecular analogs of macroscopic DNA chips. One hundred trillion probe tiles were fabricated in one step and bear pairs of 20-nucleotide-long single-stranded DNA segments that act as probe sequences. These tiles can hybridize to their targets in solution and, after adsorption onto mica surfaces, can be examined by atomic force microscopy in order to quantify binding events, because the probe segments greatly increase in stiffness upon hybridization. The nucleic acid probe tiles have been used to study position-dependent hybridization on the nanoscale and have also been used for label-free detection of RNA. 10.1126/science.1150082 KW - h KW - method AU - Ke, Yonggang AU - Lindsay, Stuart AU - Chang, Yung AU - Liu, Yan AU - Yan, Hao PY - 2008/01/11/ UR - http://dx.doi.org/10.1126/science.1150082 ER -

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