Regulation of B cell fate commitment and immunoglobulin heavy-chain gene rearrangements by Ikaros.

@article{citeulike:2966774, abstract = {The transcription factor Ikaros is essential for B cell development. However, its molecular functions in B cell fate specification and commitment have remained elusive. We show here that the transcription factor EBF restored the generation of CD19(+) pro-B cells from Ikaros-deficient hematopoietic progenitors. Notably, these pro-B cells, despite having normal expression of the transcription factors EBF and Pax5, were not committed to the B cell fate. They also failed to recombine variable gene segments at the immunoglobulin heavy-chain locus. Ikaros promoted heavy-chain gene rearrangements by inducing expression of the recombination-activating genes as well as by controlling accessibility of the variable gene segments and compaction of the immunoglobulin heavy-chain locus. Thus, Ikaros is an obligate component of a network that regulates B cell fate commitment and immunoglobulin heavy-chain gene recombination.}, address = {[1] Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois 60637, USA. [2] Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637, USA.}, author = {Reynaud, Damien and A Demarco, Ignacio and L Reddy, Karen and Schjerven, Hilde and Bertolino, Eric and Chen, Zhengshan and Smale, Stephen T T. and Winandy, Susan and Singh, Harinder }, citeulike-article-id = {2966774}, doi = {10.1038/ni.1626}, issn = {1529-2916}, journal = {Nature immunology}, keywords = {genetics, medicine, mesophysics}, month = {June}, posted-at = {2008-07-06 03:54:06}, priority = {2}, title = {Regulation of B cell fate commitment and immunoglobulin heavy-chain gene rearrangements by Ikaros.}, url = {http://dx.doi.org/10.1038/ni.1626}, year = {2008} }

TY - JOUR ID - citeulike:2966774 L3 - citeulike-article-id:2966774 TI - Regulation of B cell fate commitment and immunoglobulin heavy-chain gene rearrangements by Ikaros. JF - Nature immunology AD - [1] Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois 60637, USA. [2] Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637, USA. SN - 1529-2916 N2 - The transcription factor Ikaros is essential for B cell development. However, its molecular functions in B cell fate specification and commitment have remained elusive. We show here that the transcription factor EBF restored the generation of CD19(+) pro-B cells from Ikaros-deficient hematopoietic progenitors. Notably, these pro-B cells, despite having normal expression of the transcription factors EBF and Pax5, were not committed to the B cell fate. They also failed to recombine variable gene segments at the immunoglobulin heavy-chain locus. Ikaros promoted heavy-chain gene rearrangements by inducing expression of the recombination-activating genes as well as by controlling accessibility of the variable gene segments and compaction of the immunoglobulin heavy-chain locus. Thus, Ikaros is an obligate component of a network that regulates B cell fate commitment and immunoglobulin heavy-chain gene recombination. KW - genetics KW - medicine KW - mesophysics AU - Reynaud, Damien AU - A Demarco, Ignacio AU - L Reddy, Karen AU - Schjerven, Hilde AU - Bertolino, Eric AU - Chen, Zhengshan AU - Smale, Stephen T AU - Winandy, Susan AU - Singh, Harinder PY - 2008/06/22/ UR - http://dx.doi.org/10.1038/ni.1626 ER -