Tandem B1 elements located in a mouse methylation center provide a target for de novo DNA methylation.

@article{citeulike:2670757, abstract = {A cis-acting methylation center that signals de novo DNA methylation is located upstream of the mouse Aprt gene. In the current study, two approaches were taken to determine if tandem B1 repetitive elements found at the 3' end of the methylation center contribute to the methylation signal. First, bisulfite genomic sequencing demonstrated that CpG sites within the B1 elements were methylated at relative levels of 43\% in embryonal stem cells deficient for the maintenance DNA methyltransferase when compared with wild type embryonal stem cells. Second, the ability of the B1 elements to signal de novo methylation upon stable transfection into mouse embryonal carcinoma cells was examined. This approach demonstrated that the B1 elements were methylated de novo to a high level in the embryonal carcinoma cells and that the B1 elements acted synergistically. The results from these experiments provide strong evidence that the tandem B1 repetitive elements provide a significant fraction of the methylation center signal. By extension, they also support the hypothesis that one role for DNA methylation in mammals is to protect the genome from expression and transposition of parasitic elements.}, address = {Center for Research on Occupational and Environmental Toxicology, Oregon Health Sciences University, Portland, Oregon 97201, USA.}, author = {Yates, P. A. and Burman, R. W. and Mummaneni, P. and Krussel, S. and Turker, M. S. }, citeulike-article-id = {2670757}, issn = {0021-9258}, journal = {The Journal of biological chemistry}, keywords = {cpg\_islands}, month = {December}, number = {51}, pages = {36357--36361}, posted-at = {2008-04-14 23:35:40}, priority = {2}, title = {Tandem B1 elements located in a mouse methylation center provide a target for de novo DNA methylation.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/10593928}, volume = {274}, year = {1999} }

TY - JOUR ID - citeulike:2670757 L3 - citeulike-article-id:2670757 TI - Tandem B1 elements located in a mouse methylation center provide a target for de novo DNA methylation. JF - The Journal of biological chemistry VL - 274 IS - 51 SP - 36357 EP - 36361 AD - Center for Research on Occupational and Environmental Toxicology, Oregon Health Sciences University, Portland, Oregon 97201, USA. SN - 0021-9258 N2 - A cis-acting methylation center that signals de novo DNA methylation is located upstream of the mouse Aprt gene. In the current study, two approaches were taken to determine if tandem B1 repetitive elements found at the 3' end of the methylation center contribute to the methylation signal. First, bisulfite genomic sequencing demonstrated that CpG sites within the B1 elements were methylated at relative levels of 43% in embryonal stem cells deficient for the maintenance DNA methyltransferase when compared with wild type embryonal stem cells. Second, the ability of the B1 elements to signal de novo methylation upon stable transfection into mouse embryonal carcinoma cells was examined. This approach demonstrated that the B1 elements were methylated de novo to a high level in the embryonal carcinoma cells and that the B1 elements acted synergistically. The results from these experiments provide strong evidence that the tandem B1 repetitive elements provide a significant fraction of the methylation center signal. By extension, they also support the hypothesis that one role for DNA methylation in mammals is to protect the genome from expression and transposition of parasitic elements. KW - cpg_islands AU - Yates, PA AU - Burman, RW AU - Mummaneni, P AU - Krussel, S AU - Turker, MS PY - 1999/12/17/ UR - http://view.ncbi.nlm.nih.gov/pubmed/10593928 ER -