qPrimerDepot: a primer database for quantitative real time PCR.

by: W Cui, DD Taub, K Gardner

Nucleic acids research, Vol. 35, No. Database issue. (January 2007)

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Abstract

Gene expression studies employing high throughput real time PCR methods require finding uniform conditions for optimal amplification of multiple targets, often a daunting task. We developed a primer database, qPrimerDepot, which provides optimized primers for all human and mouse RefSeq genes. These primers are designed to amplify desired templates under unified annealing temperature. For most intron-bearing genes, primers flank one of the largest introns thus minimizing background noise due to genomic DNA contamination. The qPrimerDepot database can be accessed at http://primerdepot.nci.nih.gov/ and http://mouseprimerdepot.nci.nih.gov/.

@article{citeulike:3016380, abstract = {Gene expression studies employing high throughput real time PCR methods require finding uniform conditions for optimal amplification of multiple targets, often a daunting task. We developed a primer database, qPrimerDepot, which provides optimized primers for all human and mouse RefSeq genes. These primers are designed to amplify desired templates under unified annealing temperature. For most intron-bearing genes, primers flank one of the largest introns thus minimizing background noise due to genomic DNA contamination. The qPrimerDepot database can be accessed at http://primerdepot.nci.nih.gov/ and http://mouseprimerdepot.nci.nih.gov/.}, address = {Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, MD 20892-4605.}, author = {Cui, W. and Taub, D. D. and Gardner, K. }, citeulike-article-id = {3016380}, issn = {1362-4962}, journal = {Nucleic acids research}, keywords = {pcr, primer}, month = {January}, number = {Database issue}, posted-at = {2008-07-18 06:51:09}, priority = {2}, title = {qPrimerDepot: a primer database for quantitative real time PCR.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/17068075}, volume = {35}, year = {2007} }

RIS record

TY - JOUR ID - citeulike:3016380 L3 - citeulike-article-id:3016380 TI - qPrimerDepot: a primer database for quantitative real time PCR. JF - Nucleic acids research VL - 35 IS - Database issue AD - Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, MD 20892-4605. SN - 1362-4962 N2 - Gene expression studies employing high throughput real time PCR methods require finding uniform conditions for optimal amplification of multiple targets, often a daunting task. We developed a primer database, qPrimerDepot, which provides optimized primers for all human and mouse RefSeq genes. These primers are designed to amplify desired templates under unified annealing temperature. For most intron-bearing genes, primers flank one of the largest introns thus minimizing background noise due to genomic DNA contamination. The qPrimerDepot database can be accessed at http://primerdepot.nci.nih.gov/ and http://mouseprimerdepot.nci.nih.gov/. KW - pcr KW - primer AU - Cui, W AU - Taub, DD AU - Gardner, K PY - 2007/01// UR - http://view.ncbi.nlm.nih.gov/pubmed/17068075 ER -

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