Splicing together different regions of a gene by modified polymerase chain reaction-based site-directed mutagenesis.

@article{citeulike:2769308, abstract = {A modified polymerase chain reaction (PCR)-based site-directed mutagenesis method used to splice together different regions of a gene by deleting hundreds of nucleotides of undesired sequences is described. This method was inspired by a PCR-based site-directed mutagenesis method developed by Stratagene (La Jolla, CA, USA); the procedure and primer design were modified to enable the method to generate deletions several hundreds of nucleotides in length with an efficiency of 80-100\%, and to delete two DNA fragments simultaneously in a single PCR. This method should be useful for deletion of large DNA fragments from a gene.}, address = {Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, 200232, People's Republic of China.}, author = {Li, X. and Qiu, Y. and Shen, Y. and Ding, C. and Liu, P. and Zhou, J. and Ma, Z. }, citeulike-article-id = {2769308}, doi = {10.1016/j.ab.2007.10.021}, issn = {0003-2697}, journal = {Analytical biochemistry}, month = {February}, number = {2}, pages = {398--400}, posted-at = {2008-05-08 09:38:40}, priority = {2}, title = {Splicing together different regions of a gene by modified polymerase chain reaction-based site-directed mutagenesis.}, url = {http://dx.doi.org/10.1016/j.ab.2007.10.021}, volume = {373}, year = {2008} }

TY - JOUR ID - citeulike:2769308 L3 - citeulike-article-id:2769308 TI - Splicing together different regions of a gene by modified polymerase chain reaction-based site-directed mutagenesis. JF - Analytical biochemistry VL - 373 IS - 2 SP - 398 EP - 400 AD - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, 200232, People's Republic of China. SN - 0003-2697 N2 - A modified polymerase chain reaction (PCR)-based site-directed mutagenesis method used to splice together different regions of a gene by deleting hundreds of nucleotides of undesired sequences is described. This method was inspired by a PCR-based site-directed mutagenesis method developed by Stratagene (La Jolla, CA, USA); the procedure and primer design were modified to enable the method to generate deletions several hundreds of nucleotides in length with an efficiency of 80-100%, and to delete two DNA fragments simultaneously in a single PCR. This method should be useful for deletion of large DNA fragments from a gene. AU - Li, X AU - Qiu, Y AU - Shen, Y AU - Ding, C AU - Liu, P AU - Zhou, J AU - Ma, Z PY - 2008/02/15/ UR - http://dx.doi.org/10.1016/j.ab.2007.10.021 ER -