Physical fitness attenuates leukocyte-endothelial adhesion in response to acute exercise.

@article{citeulike:2911371, abstract = {Studies suggest that physical fitness promotes cardiovascular health, including improved endothelial function and possibly reduced inflammatory responses to stressors. This study examined the effects of fitness on leukocyte-endothelial adhesion in response to an acute exercise challenge. Peripheral blood mononuclear cell (PBMC) adhesion to human umbilical venous endothelial cells (HUVEC) was examined in 18 more-fit and 19 less-fit individuals [mean age 39 yr (SD 11)] before and after a 20-min treadmill exercise at 65-70\% peak oxygen consumption. PBMC were isolated from whole blood (Ficoll-Paque) at rest and immediately after exercise. HUVEC were incubated for 4 h in the presence of cytokines IL-1 and IL-8 to activate endothelial adhesion molecule expression. Fit subjects showed a significant reduction in PBMC-HUVEC adhesion after exercise (P < 0.01) compared with less-fit subjects, who showed no significant change. Regardless of fitness levels, both at rest and in response to exercise, soluble ICAM-1 in the incubation media attenuated PBMC-HUVEC adhesion by approximately 81\% (P < 0.001). The findings indicate that immune cells that demarginate in response to exercise have reduced ability to adhere in individuals who are physically fit, an effect apparently independent of ICAM-1 binding. The findings provide evidence of how physical fitness might protect individuals from inflammatory responses to exercise.}, address = {Department of Psychiatry, University of California, San Diego, La Jolla, CA, USA. pmills@ucsd.edu}, author = {Mills, P. J. and Hong, S. and Redwine, L. and Carter, S. M. and Chiu, A. and Ziegler, M. G. and Dimsdale, J. E. and Maisel, A. S. }, citeulike-article-id = {2911371}, doi = {10.1152/japplphysiol.00135.2006}, issn = {8750-7587}, journal = {Journal of applied physiology (Bethesda, Md. : 1985)}, keywords = {cu, exercise, leukocyte}, month = {September}, number = {3}, pages = {785--788}, posted-at = {2008-06-20 20:28:09}, priority = {0}, title = {Physical fitness attenuates leukocyte-endothelial adhesion in response to acute exercise.}, url = {http://dx.doi.org/10.1152/japplphysiol.00135.2006}, volume = {101}, year = {2006} }

TY - JOUR ID - citeulike:2911371 L3 - citeulike-article-id:2911371 TI - Physical fitness attenuates leukocyte-endothelial adhesion in response to acute exercise. JF - Journal of applied physiology (Bethesda, Md. : 1985) VL - 101 IS - 3 SP - 785 EP - 788 AD - Department of Psychiatry, University of California, San Diego, La Jolla, CA, USA. pmills@ucsd.edu SN - 8750-7587 N2 - Studies suggest that physical fitness promotes cardiovascular health, including improved endothelial function and possibly reduced inflammatory responses to stressors. This study examined the effects of fitness on leukocyte-endothelial adhesion in response to an acute exercise challenge. Peripheral blood mononuclear cell (PBMC) adhesion to human umbilical venous endothelial cells (HUVEC) was examined in 18 more-fit and 19 less-fit individuals [mean age 39 yr (SD 11)] before and after a 20-min treadmill exercise at 65-70% peak oxygen consumption. PBMC were isolated from whole blood (Ficoll-Paque) at rest and immediately after exercise. HUVEC were incubated for 4 h in the presence of cytokines IL-1 and IL-8 to activate endothelial adhesion molecule expression. Fit subjects showed a significant reduction in PBMC-HUVEC adhesion after exercise (P < 0.01) compared with less-fit subjects, who showed no significant change. Regardless of fitness levels, both at rest and in response to exercise, soluble ICAM-1 in the incubation media attenuated PBMC-HUVEC adhesion by approximately 81% (P < 0.001). The findings indicate that immune cells that demarginate in response to exercise have reduced ability to adhere in individuals who are physically fit, an effect apparently independent of ICAM-1 binding. The findings provide evidence of how physical fitness might protect individuals from inflammatory responses to exercise. KW - cu KW - exercise KW - leukocyte AU - Mills, PJ AU - Hong, S AU - Redwine, L AU - Carter, SM AU - Chiu, A AU - Ziegler, MG AU - Dimsdale, JE AU - Maisel, AS PY - 2006/09// UR - http://dx.doi.org/10.1152/japplphysiol.00135.2006 ER -