Quantitative and predictive model of transcriptional control of the Drosophila melanogaster even skipped gene.

by: Hilde Janssens, Shuling Hou, Johannes Jaeger, Ah-Ram R Kim, Ekaterina Myasnikova, David Sharp, John Reinitz

Nat Genet (17 September 2006)

View FullText article

DOI, Nature, Pubmed, Hubmed

Reviews [Write a review of this article]

There are no reviews of this article

Find related articles from these CiteULike users

okarsligil, brant, dpollard, adepace, allPowerde, Stew, Bioinformatics (group), EisenLab (group), emptyhb

Find related articles with these CiteULike tags

cis-regulatory, cis_regulatory_elements, dmel_mse2_regulatory_model, drosophila, embryo, even-skipped_enhancer, expression, gene, key--crm, key--expression_pattern, model, modeling_gene_network, no-tag, pattern, prediction, predictive, system--dmel_embryo, systems-biology, tech--network_modeling, tech--quantitative_imaging, transcription

Abstract

Here we present a quantitative and predictive model of the transcriptional readout of the proximal 1.7 kb of the control region of the Drosophila melanogaster gene even skipped (eve). The model is based on the positions and sequence of individual binding sites on the DNA and quantitative, time-resolved expression data at cellular resolution. These data demonstrated new expression features, first reported here. The model correctly predicts the expression patterns of mutations in trans, as well as point mutations, insertions and deletions in cis. It also shows that the nonclassical expression of stripe 7 driven by this fragment is activated by the protein Caudal (Cad), and repressed by the proteins Tailless (Tll) and Giant (Gt).

@article{citeulike:874325, abstract = {Here we present a quantitative and predictive model of the transcriptional readout of the proximal 1.7 kb of the control region of the Drosophila melanogaster gene even skipped (eve). The model is based on the positions and sequence of individual binding sites on the DNA and quantitative, time-resolved expression data at cellular resolution. These data demonstrated new expression features, first reported here. The model correctly predicts the expression patterns of mutations in trans, as well as point mutations, insertions and deletions in cis. It also shows that the nonclassical expression of stripe 7 driven by this fragment is activated by the protein Caudal (Cad), and repressed by the proteins Tailless (Tll) and Giant (Gt).}, address = {Department of Applied Mathematics and Statistics, and Center for Developmental Genetics, Stony Brook University, Stony Brook, New York 11794-3600, USA.}, author = {Janssens, Hilde and Hou, Shuling and Jaeger, Johannes and Kim, Ah-Ram R. and Myasnikova, Ekaterina and Sharp, David and Reinitz, John }, citeulike-article-id = {874325}, doi = {10.1038/ng1886}, issn = {1061-4036}, journal = {Nat Genet}, keywords = {cis\_regulatory\_elements, drosophila, even-skipped\_enhancer, modeling\_gene\_network}, month = {September}, posted-at = {2008-01-15 20:48:34}, priority = {2}, title = {Quantitative and predictive model of transcriptional control of the Drosophila melanogaster even skipped gene.}, url = {http://dx.doi.org/10.1038/ng1886}, year = {2006} }

RIS record

TY - JOUR ID - citeulike:874325 L3 - citeulike-article-id:874325 TI - Quantitative and predictive model of transcriptional control of the Drosophila melanogaster even skipped gene. JF - Nat Genet AD - Department of Applied Mathematics and Statistics, and Center for Developmental Genetics, Stony Brook University, Stony Brook, New York 11794-3600, USA. SN - 1061-4036 N2 - Here we present a quantitative and predictive model of the transcriptional readout of the proximal 1.7 kb of the control region of the Drosophila melanogaster gene even skipped (eve). The model is based on the positions and sequence of individual binding sites on the DNA and quantitative, time-resolved expression data at cellular resolution. These data demonstrated new expression features, first reported here. The model correctly predicts the expression patterns of mutations in trans, as well as point mutations, insertions and deletions in cis. It also shows that the nonclassical expression of stripe 7 driven by this fragment is activated by the protein Caudal (Cad), and repressed by the proteins Tailless (Tll) and Giant (Gt). KW - cis_regulatory_elements KW - drosophila KW - even-skipped_enhancer KW - modeling_gene_network AU - Janssens, Hilde AU - Hou, Shuling AU - Jaeger, Johannes AU - Kim, Ah-Ram AU - Myasnikova, Ekaterina AU - Sharp, David AU - Reinitz, John PY - 2006/09/17/ UR - http://dx.doi.org/10.1038/ng1886 ER -

RIS BibTeX