A comparison of in vivo and in vitro DNA-binding specificities suggests a new model for homeoprotein DNA binding in Drosophila embryos.

@article{citeulike:2647530, abstract = {Little is known about the range of DNA sequences bound by transcription factors in vivo. Using a sensitive UV cross-linking technique, we show that three classes of homeoprotein bind at significant levels to the majority of genes in Drosophila embryos. The three classes bind with specificities different from each other; however, their levels of binding on any single DNA fragment differ by no more than 5- to 10-fold. On actively transcribed genes, there is a good correlation between the in vivo DNA-binding specificity of each class and its in vitro DNA-binding specificity. In contrast, no such correlation is seen on inactive or weakly transcribed genes. These genes are bound poorly in vivo, even though they contain many high affinity homeoprotein-binding sites. Based on these results, we suggest how the in vivo pattern of homeoprotein DNA binding is determined.}, address = {Department of Molecular Biophysics and Biochemistry, Yale University, PO Box 208114, New Haven, CT 06520-8114, USA.}, author = {Carr, A. and Biggin, M. D. }, citeulike-article-id = {2647530}, issn = {0261-4189}, journal = {The EMBO journal}, keywords = {dna-protein\_interaction, in-vivo\_binding\_sites}, month = {March}, number = {6}, pages = {1598--1608}, posted-at = {2008-04-10 00:46:32}, priority = {2}, title = {A comparison of in vivo and in vitro DNA-binding specificities suggests a new model for homeoprotein DNA binding in Drosophila embryos.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/10075930}, volume = {18}, year = {1999} }

TY - JOUR ID - citeulike:2647530 L3 - citeulike-article-id:2647530 TI - A comparison of in vivo and in vitro DNA-binding specificities suggests a new model for homeoprotein DNA binding in Drosophila embryos. JF - The EMBO journal VL - 18 IS - 6 SP - 1598 EP - 1608 AD - Department of Molecular Biophysics and Biochemistry, Yale University, PO Box 208114, New Haven, CT 06520-8114, USA. SN - 0261-4189 N2 - Little is known about the range of DNA sequences bound by transcription factors in vivo. Using a sensitive UV cross-linking technique, we show that three classes of homeoprotein bind at significant levels to the majority of genes in Drosophila embryos. The three classes bind with specificities different from each other; however, their levels of binding on any single DNA fragment differ by no more than 5- to 10-fold. On actively transcribed genes, there is a good correlation between the in vivo DNA-binding specificity of each class and its in vitro DNA-binding specificity. In contrast, no such correlation is seen on inactive or weakly transcribed genes. These genes are bound poorly in vivo, even though they contain many high affinity homeoprotein-binding sites. Based on these results, we suggest how the in vivo pattern of homeoprotein DNA binding is determined. KW - dna-protein_interaction KW - in-vivo_binding_sites AU - Carr, A AU - Biggin, MD PY - 1999/03/15/ UR - http://view.ncbi.nlm.nih.gov/pubmed/10075930 ER -