Quantitative specificity of the Mnt repressor.

@article{citeulike:2633498, abstract = {The Mnt protein of Salmonella phage P22 binds site-specifically to its operator. To better understand this binding we used dideoxy DNA sequencing in a quantitative manner to determine the relative binding constants, and hence the relative free energies, of wild-type Mnt protein to a substantial number of variants of its operator. These measurements were supported by experiments which used the SELEX procedure to generate a set of operators from an initially randomized population. In the Discussion we show that the present model of Mnt protein/operator binding, due to Sauer and co-workers, along with the assumption of an independent contribution of each position in the operator to the total binding, provides a reasonably accurate description of the system. We also discuss the use of information content as a measure of DNA-protein binding specificity with the Mnt protein/operator system serving as an example and show again that the assumption of independence supports the current view of this case of site-specific binding.}, address = {Department of Molecular Cellular, and Developmental Biology, University of Colorado at Boulder, Boulder, CO 80309-0347, USA.}, author = {Fields, D. S. and He, Y. and Al-Uzri, A. Y. and Stormo, G. D. }, citeulike-article-id = {2633498}, issn = {0022-2836}, journal = {Journal of molecular biology}, keywords = {biophysics, cis\_regulatory\_elements, dna-protein\_interaction}, month = {August}, number = {2}, pages = {178--194}, posted-at = {2008-04-05 19:56:01}, priority = {2}, title = {Quantitative specificity of the Mnt repressor.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9268651}, volume = {271}, year = {1997} }

TY - JOUR ID - citeulike:2633498 L3 - citeulike-article-id:2633498 TI - Quantitative specificity of the Mnt repressor. JF - Journal of molecular biology VL - 271 IS - 2 SP - 178 EP - 194 AD - Department of Molecular Cellular, and Developmental Biology, University of Colorado at Boulder, Boulder, CO 80309-0347, USA. SN - 0022-2836 N2 - The Mnt protein of Salmonella phage P22 binds site-specifically to its operator. To better understand this binding we used dideoxy DNA sequencing in a quantitative manner to determine the relative binding constants, and hence the relative free energies, of wild-type Mnt protein to a substantial number of variants of its operator. These measurements were supported by experiments which used the SELEX procedure to generate a set of operators from an initially randomized population. In the Discussion we show that the present model of Mnt protein/operator binding, due to Sauer and co-workers, along with the assumption of an independent contribution of each position in the operator to the total binding, provides a reasonably accurate description of the system. We also discuss the use of information content as a measure of DNA-protein binding specificity with the Mnt protein/operator system serving as an example and show again that the assumption of independence supports the current view of this case of site-specific binding. KW - biophysics KW - cis_regulatory_elements KW - dna-protein_interaction AU - Fields, DS AU - He, Y AU - Al-Uzri, AY AU - Stormo, GD PY - 1997/08/15/ UR - http://view.ncbi.nlm.nih.gov/pubmed/9268651 ER -