FoxA1 translates epigenetic signatures into enhancer-driven lineage-specific transcription.

@article{citeulike:2746814, abstract = {Complex organisms require tissue-specific transcriptional programs, yet little is known about how these are established. The transcription factor FoxA1 is thought to contribute to gene regulation through its ability to act as a pioneer factor binding to nucleosomal DNA. Through genome-wide positional analyses, we demonstrate that FoxA1 cell type-specific functions rely primarily on differential recruitment to chromatin predominantly at distant enhancers rather than proximal promoters. This differential recruitment leads to cell type-specific changes in chromatin structure and functional collaboration with lineage-specific transcription factors. Despite the ability of FoxA1 to bind nucleosomes, its differential binding to chromatin sites is dependent on the distribution of histone H3 lysine 4 dimethylation. Together, our results suggest that methylation of histone H3 lysine 4 is part of the epigenetic signature that defines lineage-specific FoxA1 recruitment sites in chromatin. FoxA1 translates this epigenetic signature into changes in chromatin structure thereby establishing lineage-specific transcriptional enhancers and programs.}, address = {Division of Molecular and Cellular Oncology, Department of Medical Oncology, Dana-Farber Cancer Institute and Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA.}, author = {Lupien, M. and Eeckhoute, J. and Meyer, C. A. and Wang, Q. and Zhang, Y. and Li, W. and Carroll, J. S. and Liu, X. S. and Brown, M. }, citeulike-article-id = {2746814}, doi = {10.1016/j.cell.2008.01.018}, issn = {1097-4172}, journal = {Cell}, keywords = {histones}, month = {March}, number = {6}, pages = {958--970}, posted-at = {2008-05-02 19:48:31}, priority = {2}, title = {FoxA1 translates epigenetic signatures into enhancer-driven lineage-specific transcription.}, url = {http://dx.doi.org/10.1016/j.cell.2008.01.018}, volume = {132}, year = {2008} }

TY - JOUR ID - citeulike:2746814 L3 - citeulike-article-id:2746814 TI - FoxA1 translates epigenetic signatures into enhancer-driven lineage-specific transcription. JF - Cell VL - 132 IS - 6 SP - 958 EP - 970 AD - Division of Molecular and Cellular Oncology, Department of Medical Oncology, Dana-Farber Cancer Institute and Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA. SN - 1097-4172 N2 - Complex organisms require tissue-specific transcriptional programs, yet little is known about how these are established. The transcription factor FoxA1 is thought to contribute to gene regulation through its ability to act as a pioneer factor binding to nucleosomal DNA. Through genome-wide positional analyses, we demonstrate that FoxA1 cell type-specific functions rely primarily on differential recruitment to chromatin predominantly at distant enhancers rather than proximal promoters. This differential recruitment leads to cell type-specific changes in chromatin structure and functional collaboration with lineage-specific transcription factors. Despite the ability of FoxA1 to bind nucleosomes, its differential binding to chromatin sites is dependent on the distribution of histone H3 lysine 4 dimethylation. Together, our results suggest that methylation of histone H3 lysine 4 is part of the epigenetic signature that defines lineage-specific FoxA1 recruitment sites in chromatin. FoxA1 translates this epigenetic signature into changes in chromatin structure thereby establishing lineage-specific transcriptional enhancers and programs. KW - histones AU - Lupien, M AU - Eeckhoute, J AU - Meyer, CA AU - Wang, Q AU - Zhang, Y AU - Li, W AU - Carroll, JS AU - Liu, XS AU - Brown, M PY - 2008/03/21/ UR - http://dx.doi.org/10.1016/j.cell.2008.01.018 ER -