Global Analysis of mRNA Localization Reveals a Prominent Role in Organizing Cellular Architecture and Function

@article{citeulike:1732275, abstract = {Summary Although subcellular mRNA trafficking has been demonstrated as a mechanism to control protein distribution, it is generally believed that most protein localization occurs subsequent to translation. To address this point, we developed and employed a high-resolution fluorescent in situ hybridization procedure to comprehensively evaluate mRNA localization dynamics during early Drosophila embryogenesis. Surprisingly, of the 3370 genes analyzed, 71\% of those expressed encode subcellularly localized mRNAs. Dozens of new and striking localization patterns were observed, implying an equivalent variety of localization mechanisms. Tight correlations between mRNA distribution and subsequent protein localization and function, indicate major roles for mRNA localization in nucleating localized cellular machineries. A searchable web resource documenting mRNA expression and localization dynamics has been established and will serve as an invaluable tool for dissecting localization mechanisms and for predicting gene functions and interactions.}, author = {Lecuyer, Eric and Yoshida, Hideki and Parthasarathy, Neela and Alm, Christina and Babak, Tomas and Cerovina, Tanja and Hughes, Timothy R. and Tomancak, Pavel and Krause, Henry M. }, citeulike-article-id = {1732275}, doi = {http://dx.doi.org/10.1016/j.cell.2007.08.003}, journal = {Cell}, keywords = {drosophila, gene\_expression, in\_situ}, month = {October}, number = {1}, pages = {174--187}, posted-at = {2007-10-19 23:10:47}, priority = {3}, title = {Global Analysis of mRNA Localization Reveals a Prominent Role in Organizing Cellular Architecture and Function}, url = {http://dx.doi.org/10.1016/j.cell.2007.08.003}, volume = {131}, year = {2007} }

TY - JOUR ID - citeulike:1732275 L3 - citeulike-article-id:1732275 TI - Global Analysis of mRNA Localization Reveals a Prominent Role in Organizing Cellular Architecture and Function JF - Cell VL - 131 IS - 1 SP - 174 EP - 187 N2 - Summary Although subcellular mRNA trafficking has been demonstrated as a mechanism to control protein distribution, it is generally believed that most protein localization occurs subsequent to translation. To address this point, we developed and employed a high-resolution fluorescent in situ hybridization procedure to comprehensively evaluate mRNA localization dynamics during early Drosophila embryogenesis. Surprisingly, of the 3370 genes analyzed, 71% of those expressed encode subcellularly localized mRNAs. Dozens of new and striking localization patterns were observed, implying an equivalent variety of localization mechanisms. Tight correlations between mRNA distribution and subsequent protein localization and function, indicate major roles for mRNA localization in nucleating localized cellular machineries. A searchable web resource documenting mRNA expression and localization dynamics has been established and will serve as an invaluable tool for dissecting localization mechanisms and for predicting gene functions and interactions. KW - drosophila KW - gene_expression KW - in_situ AU - Lecuyer, Eric AU - Yoshida, Hideki AU - Parthasarathy, Neela AU - Alm, Christina AU - Babak, Tomas AU - Cerovina, Tanja AU - Hughes, Timothy AU - Tomancak, Pavel AU - Krause, Henry PY - 2007/10/05/ UR - http://dx.doi.org/10.1016/j.cell.2007.08.003 ER -