Global eQTL Mapping Reveals the Complex Genetic Architecture of Transcript-Level Variation in Arabidopsis

@article{citeulike:1187570, abstract = {The genetic architecture of transcript-level variation is largely unknown. The genetic determinants of transcript-level variation were characterized in a recombinant inbred line (RIL) population (n = 211) of Arabidopsis thaliana using whole-genome microarray analysis and expression quantitative trait loci (eQTL) mapping of transcript levels as expression traits (e-traits). Genetic control of transcription was highly complex: one-third of the quantitatively controlled transcripts/e-traits were regulated by cis-eQTL, and many trans-eQTL mapped to hotspots that regulated hundreds to thousands of e-traits. Several thousand eQTL of large phenotypic effect were detected, but almost all (93\%) of the 36,871 eQTL were associated with small phenotypic effects (R2 < 0.3). Many transcripts/e-traits were controlled by multiple eQTL with opposite allelic effects and exhibited higher heritability in the RILs than their parents, suggesting nonadditive genetic variation. To our knowledge, this is the first large-scale global eQTL study in a relatively large plant mapping population. It reveals that the genetic control of transcript level is highly variable and multifaceted and that this complexity may be a general characteristic of eukaryotes. 10.1534/genetics.106.064972}, author = {West, Marilyn A. and Kim, Kyunga and Kliebenstein, Daniel J. and van Leeuwen, Hans and Michelmore, Richard W. and Doerge, R. W. and St. Clair, Dina A. }, citeulike-article-id = {1187570}, doi = {10.1534/genetics.106.064972}, journal = {Genetics}, keywords = {eqtl, plants}, month = {March}, number = {3}, pages = {1441--1450}, posted-at = {2007-03-26 08:14:33}, priority = {3}, title = {Global eQTL Mapping Reveals the Complex Genetic Architecture of Transcript-Level Variation in Arabidopsis}, url = {http://dx.doi.org/10.1534/genetics.106.064972}, volume = {175}, year = {2007} }

TY - JOUR ID - citeulike:1187570 L3 - citeulike-article-id:1187570 TI - Global eQTL Mapping Reveals the Complex Genetic Architecture of Transcript-Level Variation in Arabidopsis JF - Genetics VL - 175 IS - 3 SP - 1441 EP - 1450 N2 - The genetic architecture of transcript-level variation is largely unknown. The genetic determinants of transcript-level variation were characterized in a recombinant inbred line (RIL) population (n = 211) of Arabidopsis thaliana using whole-genome microarray analysis and expression quantitative trait loci (eQTL) mapping of transcript levels as expression traits (e-traits). Genetic control of transcription was highly complex: one-third of the quantitatively controlled transcripts/e-traits were regulated by cis-eQTL, and many trans-eQTL mapped to hotspots that regulated hundreds to thousands of e-traits. Several thousand eQTL of large phenotypic effect were detected, but almost all (93%) of the 36,871 eQTL were associated with small phenotypic effects (R2 < 0.3). Many transcripts/e-traits were controlled by multiple eQTL with opposite allelic effects and exhibited higher heritability in the RILs than their parents, suggesting nonadditive genetic variation. To our knowledge, this is the first large-scale global eQTL study in a relatively large plant mapping population. It reveals that the genetic control of transcript level is highly variable and multifaceted and that this complexity may be a general characteristic of eukaryotes. 10.1534/genetics.106.064972 KW - eqtl KW - plants AU - West, Marilyn AU - Kim, Kyunga AU - Kliebenstein, Daniel AU - van Leeuwen, Hans AU - Michelmore, Richard AU - Doerge, RW AU - St. Clair, Dina PY - 2007/03/01/ UR - http://dx.doi.org/10.1534/genetics.106.064972 ER -