Whole-genome ChIP-chip analysis of Dorsal, Twist, and Snail suggests integration of diverse patterning processes in the Drosophila embryo.

@article{citeulike:1161647, abstract = {Genetic studies have identified numerous sequence-specific transcription factors that control development, yet little is known about their in vivo distribution across animal genomes. We determined the genome-wide occupancy of the dorsoventral (DV) determinants Dorsal, Twist, and Snail in the Drosophila embryo using chromatin immunoprecipitation coupled with microarray analysis (ChIP-chip). The in vivo binding of these proteins correlate tightly with the limits of known enhancers. Our analysis predicts substantially more target genes than previous estimates, and includes Dpp signaling components and anteroposterior (AP) segmentation determinants. Thus, the ChIP-chip data uncover a much larger than expected regulatory network, which integrates diverse patterning processes during development.}, address = {Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA.}, author = {Zeitlinger, J. and Zinzen, R. P. and Stark, A. and Kellis, M. and Zhang, H. and Young, R. A. and Levine, M. }, citeulike-article-id = {1161647}, doi = {10.1101/gad.1509607}, issn = {0890-9369}, journal = {Genes Dev}, keywords = {ap\_x\_dv, chip\_chip, drosophila}, month = {February}, number = {4}, pages = {385--390}, posted-at = {2007-03-23 01:04:22}, priority = {3}, title = {Whole-genome ChIP-chip analysis of Dorsal, Twist, and Snail suggests integration of diverse patterning processes in the Drosophila embryo.}, url = {http://dx.doi.org/10.1101/gad.1509607}, volume = {21}, year = {2007} }

TY - JOUR ID - citeulike:1161647 L3 - citeulike-article-id:1161647 TI - Whole-genome ChIP-chip analysis of Dorsal, Twist, and Snail suggests integration of diverse patterning processes in the Drosophila embryo. JF - Genes Dev VL - 21 IS - 4 SP - 385 EP - 390 AD - Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA. SN - 0890-9369 N2 - Genetic studies have identified numerous sequence-specific transcription factors that control development, yet little is known about their in vivo distribution across animal genomes. We determined the genome-wide occupancy of the dorsoventral (DV) determinants Dorsal, Twist, and Snail in the Drosophila embryo using chromatin immunoprecipitation coupled with microarray analysis (ChIP-chip). The in vivo binding of these proteins correlate tightly with the limits of known enhancers. Our analysis predicts substantially more target genes than previous estimates, and includes Dpp signaling components and anteroposterior (AP) segmentation determinants. Thus, the ChIP-chip data uncover a much larger than expected regulatory network, which integrates diverse patterning processes during development. KW - ap_x_dv KW - chip_chip KW - drosophila AU - Zeitlinger, J AU - Zinzen, RP AU - Stark, A AU - Kellis, M AU - Zhang, H AU - Young, RA AU - Levine, M PY - 2007/02/15/ UR - http://dx.doi.org/10.1101/gad.1509607 ER -