Structure-based prediction of C2H2 zinc-finger binding specificity: sensitivity to docking geometry.

@article{citeulike:1095919, abstract = {Predicting the binding specificity of transcription factors is a critical step in the characterization and computational identification and of cis-regulatory elements in genomic sequences. Here we use protein-DNA structures to predict binding specificity and consider the possibility of predicting position weight matrices (PWM) for an entire protein family based on the structures of just a few family members. A particular focus is the sensitivity of prediction accuracy to the docking geometry of the structure used. We investigate this issue with the goal of determining how similar two docking geometries must be for binding specificity predictions to be accurate. Docking similarity is quantified using our recently described interface alignment score (IAS). Using a molecular-mechanics force field, we predict high-affinity nucleotide sequences that bind to the second zinc-finger (ZF) domain from the Zif268 protein, using different C(2)H(2) ZF domains as structural templates. We identify a strong relationship between IAS values and prediction accuracy, and define a range of IAS values for which accurate structure-based predictions of binding specificity is to be expected. The implication of our results for large-scale, structure-based prediction of PWMs is discussed.}, address = {Howard Hughes Medical Institute, Center for Computational Biology and Bioinformatics, Department of Biochemistry and Molecular Biophysics, Columbia University, 1130 St. Nicholas Avenue, Room 815, New York, NY 10032, USA.}, author = {Siggers, Trevor W W. and Honig, Barry }, citeulike-article-id = {1095919}, issn = {1362-4962}, journal = {Nucleic Acids Res}, keywords = {affinity, binding\_site, dan\_presented, eisen\_journal\_club, prediction, round\_robin, structure, transcription\_factor}, month = {January}, posted-at = {2007-02-09 01:34:13}, priority = {0}, title = {Structure-based prediction of C2H2 zinc-finger binding specificity: sensitivity to docking geometry.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/17264128}, year = {2007} }

TY - JOUR ID - citeulike:1095919 L3 - citeulike-article-id:1095919 TI - Structure-based prediction of C2H2 zinc-finger binding specificity: sensitivity to docking geometry. JF - Nucleic Acids Res AD - Howard Hughes Medical Institute, Center for Computational Biology and Bioinformatics, Department of Biochemistry and Molecular Biophysics, Columbia University, 1130 St. Nicholas Avenue, Room 815, New York, NY 10032, USA. SN - 1362-4962 N2 - Predicting the binding specificity of transcription factors is a critical step in the characterization and computational identification and of cis-regulatory elements in genomic sequences. Here we use protein-DNA structures to predict binding specificity and consider the possibility of predicting position weight matrices (PWM) for an entire protein family based on the structures of just a few family members. A particular focus is the sensitivity of prediction accuracy to the docking geometry of the structure used. We investigate this issue with the goal of determining how similar two docking geometries must be for binding specificity predictions to be accurate. Docking similarity is quantified using our recently described interface alignment score (IAS). Using a molecular-mechanics force field, we predict high-affinity nucleotide sequences that bind to the second zinc-finger (ZF) domain from the Zif268 protein, using different C(2)H(2) ZF domains as structural templates. We identify a strong relationship between IAS values and prediction accuracy, and define a range of IAS values for which accurate structure-based predictions of binding specificity is to be expected. The implication of our results for large-scale, structure-based prediction of PWMs is discussed. KW - affinity KW - binding_site KW - dan_presented KW - eisen_journal_club KW - prediction KW - round_robin KW - structure KW - transcription_factor AU - Siggers, Trevor W AU - Honig, Barry PY - 2007/01/30/ UR - http://view.ncbi.nlm.nih.gov/pubmed/17264128 ER -