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N-Hydroxysuccinimide ester labeling 5'-aminoalkyl DNA oligomers: reaction conditions and purification

by: Zhixian Wang, Nanying Bian, Xiaohua Qian, Roger W Giese
Journal of Chromatography A, Vol. 806, No. 1. (8 May 1998), pp. 93-95.

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Difficulties were encountered in labeling 5'-aminoalkyl DNA oligomers with glycolketo electrophore N-hydroxysuccinimide esters in aqueous sodium bicarbonate (a common base for this purpose), followed by C18-silica reversed-phase high-performance liquid chromatography (HPLC) to achieve purification. The electrophore-labeled oligomers were not separated readily either from the hydrolyzed electrophore or from the starting oligomer. This problem was overcome by conducting the reaction with triethylamine as a base, organic washing the reaction mixtures after evaporation, and separating on a C18-poly(styrene-divinylbenzene) HPLC packing.


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