Nucleosome organization in the Drosophila genome.

@article{citeulike:2679115, abstract = {Comparative genomics of nucleosome positions provides a powerful means for understanding how the organization of chromatin and the transcription machinery co-evolve. Here we produce a high-resolution reference map of H2A.Z and bulk nucleosome locations across the genome of the fly Drosophila melanogaster and compare it to that from the yeast Saccharomyces cerevisiae. Like Saccharomyces, Drosophila nucleosomes are organized around active transcription start sites in a canonical -1, nucleosome-free region, +1 arrangement. However, Drosophila does not incorporate H2A.Z into the -1 nucleosome and does not bury its transcriptional start site in the +1 nucleosome. At thousands of genes, RNA polymerase II engages the +1 nucleosome and pauses. How the transcription initiation machinery contends with the +1 nucleosome seems to be fundamentally different across major eukaryotic lines.}, address = {[1] Center for Gene Regulation, and, [2] Center for Comparative Genomics and Bioinformatics, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA [3] These authors contributed equally to this work.}, author = {Mavrich, Travis N N. and Jiang, Cizhong and Ioshikhes, Ilya P P. and Li, Xiaoyong and Venters, Bryan J J. and Zanton, Sara J J. and Tomsho, Lynn P P. and Qi, Ji and Glaser, Robert L L. and Schuster, Stephan C C. and Gilmour, David S S. and Albert, Istvan and Pugh, B Franklin F. }, citeulike-article-id = {2679115}, doi = {10.1038/nature06929}, issn = {1476-4687}, journal = {Nature}, keywords = {chromatin, fly}, month = {April}, posted-at = {2008-05-14 21:05:54}, priority = {2}, title = {Nucleosome organization in the Drosophila genome.}, url = {http://dx.doi.org/10.1038/nature06929}, year = {2008} }

TY - JOUR ID - citeulike:2679115 L3 - citeulike-article-id:2679115 TI - Nucleosome organization in the Drosophila genome. JF - Nature AD - [1] Center for Gene Regulation, and, [2] Center for Comparative Genomics and Bioinformatics, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA [3] These authors contributed equally to this work. SN - 1476-4687 N2 - Comparative genomics of nucleosome positions provides a powerful means for understanding how the organization of chromatin and the transcription machinery co-evolve. Here we produce a high-resolution reference map of H2A.Z and bulk nucleosome locations across the genome of the fly Drosophila melanogaster and compare it to that from the yeast Saccharomyces cerevisiae. Like Saccharomyces, Drosophila nucleosomes are organized around active transcription start sites in a canonical -1, nucleosome-free region, +1 arrangement. However, Drosophila does not incorporate H2A.Z into the -1 nucleosome and does not bury its transcriptional start site in the +1 nucleosome. At thousands of genes, RNA polymerase II engages the +1 nucleosome and pauses. How the transcription initiation machinery contends with the +1 nucleosome seems to be fundamentally different across major eukaryotic lines. KW - chromatin KW - fly AU - Mavrich, Travis N AU - Jiang, Cizhong AU - Ioshikhes, Ilya P AU - Li, Xiaoyong AU - Venters, Bryan J AU - Zanton, Sara J AU - Tomsho, Lynn P AU - Qi, Ji AU - Glaser, Robert L AU - Schuster, Stephan C AU - Gilmour, David S AU - Albert, Istvan AU - Pugh, B Franklin PY - 2008/04/13/ UR - http://dx.doi.org/10.1038/nature06929 ER -