Repression of deoP2 in Escherichia coli by CytR: conversion of a transcription activator into a repressor.

@article{citeulike:1320138, abstract = {In the deoP2 promoter of Escherichia coli, a transcription activator, cAMP-CRP, binds at two sites, centered at -41.5 and -93.5 from the start site of transcription, while a repressor, CytR, binds to a space between the two cAMP-CRP complexes. The mechanisms for the cAMP-CRP-mediated transcription activation and CytR-mediated transcription repression were investigated in vitro using purified components. We classified the deoP2 promoter as a class II cAMP-CRP-dependent promoter, primarily by the action of cAMP-CRP at the downstream site. Interestingly, we also found that deoP2 carries an "UP-element" immediately upstream of the downstream cAMP-CRP site. The UP-element overlaps with the DNA site for CytR. However, it was observed that CytR functions with the RNA polymerase devoid of the C-terminal domain of the alpha-subunit as well as with intact RNA polymerase. The mechanism of repression by CytR proposed in this study is that the cAMP-CRP bound at -41.5 undergoes an allosteric change upon direct interaction with CytR such that it no longer maintains a productive interaction with the N-terminal domain of alpha, but instead acts as a repressor to interfere with RNA polymerase acting on deoP2.}, address = {Department of Microbiology, Chonnam University Medical College, 5 Hakdong, Dongku, Gwangju, South Korea 501-714.}, author = {Shin, M. and Kang, S. and Hyun, S. J. and Fujita, N. and Ishihama, A. and Valentin-Hansen, P. and Choy, H. E. }, citeulike-article-id = {1320138}, issn = {0261-4189}, journal = {EMBO J}, month = {October}, number = {19}, pages = {5392--5399}, posted-at = {2007-05-23 00:29:16}, priority = {1}, title = {Repression of deoP2 in Escherichia coli by CytR: conversion of a transcription activator into a repressor.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/11574471}, volume = {20}, year = {2001} }

TY - JOUR ID - citeulike:1320138 L3 - citeulike-article-id:1320138 TI - Repression of deoP2 in Escherichia coli by CytR: conversion of a transcription activator into a repressor. JF - EMBO J VL - 20 IS - 19 SP - 5392 EP - 5399 AD - Department of Microbiology, Chonnam University Medical College, 5 Hakdong, Dongku, Gwangju, South Korea 501-714. SN - 0261-4189 N2 - In the deoP2 promoter of Escherichia coli, a transcription activator, cAMP-CRP, binds at two sites, centered at -41.5 and -93.5 from the start site of transcription, while a repressor, CytR, binds to a space between the two cAMP-CRP complexes. The mechanisms for the cAMP-CRP-mediated transcription activation and CytR-mediated transcription repression were investigated in vitro using purified components. We classified the deoP2 promoter as a class II cAMP-CRP-dependent promoter, primarily by the action of cAMP-CRP at the downstream site. Interestingly, we also found that deoP2 carries an "UP-element" immediately upstream of the downstream cAMP-CRP site. The UP-element overlaps with the DNA site for CytR. However, it was observed that CytR functions with the RNA polymerase devoid of the C-terminal domain of the alpha-subunit as well as with intact RNA polymerase. The mechanism of repression by CytR proposed in this study is that the cAMP-CRP bound at -41.5 undergoes an allosteric change upon direct interaction with CytR such that it no longer maintains a productive interaction with the N-terminal domain of alpha, but instead acts as a repressor to interfere with RNA polymerase acting on deoP2. AU - Shin, M AU - Kang, S AU - Hyun, SJ AU - Fujita, N AU - Ishihama, A AU - Valentin-Hansen, P AU - Choy, HE PY - 2001/10/01/ UR - http://view.ncbi.nlm.nih.gov/pubmed/11574471 ER -