Silencing of African horse sickness virus VP7 protein expression in cultured cells by RNA interference.

@article{citeulike:1657209, abstract = {RNA interference (RNAi) is the process by which double-stranded RNA directs sequence-specific degradation of homologous mRNA. Short interfering RNAs (siRNAs) are the mediators of RNAi and represent powerful tools to silence gene expression in mammalian cells including genes of viral origin. In this study, we applied siRNAs targeting the VP7 gene of African horse sickness virus (AHSV) that encodes a structural protein required for stable capsid assembly. Using a VP7 expression reporter plasmid and an in vitro model of infection, we show that synthetic siRNA molecules corresponding to the AHSV VP7 gene silenced effectively VP7 protein and mRNA expression, and decreased production of infectious virus particles as evidenced by a reduction in the progeny virion titres when compared to control cells. This work establishes RNAi as a genetic tool for the study of AHSV and offers new possibilities for the analysis of viral genes important for AHSV physiology.}, address = {Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria, 0002, South Africa, lieselburger@tuks.co.za.}, author = {Stassen, Liesel and Huismans, Henk and Theron, Jacques }, citeulike-article-id = {1657209}, doi = {http://dx.doi.org/10.1007/s11262-007-0162-4}, issn = {0920-8569}, journal = {Virus Genes}, keywords = {african-horse-sickness, orbivirus, rnai}, month = {September}, posted-at = {2007-09-14 16:10:33}, priority = {2}, title = {Silencing of African horse sickness virus VP7 protein expression in cultured cells by RNA interference.}, url = {http://dx.doi.org/10.1007/s11262-007-0162-4}, year = {2007} }

TY - JOUR ID - citeulike:1657209 L3 - citeulike-article-id:1657209 N2 - RNA interference (RNAi) is the process by which double-stranded RNA directs sequence-specific degradation of homologous mRNA. Short interfering RNAs (siRNAs) are the mediators of RNAi and represent powerful tools to silence gene expression in mammalian cells including genes of viral origin. In this study, we applied siRNAs targeting the VP7 gene of African horse sickness virus (AHSV) that encodes a structural protein required for stable capsid assembly. Using a VP7 expression reporter plasmid and an in vitro model of infection, we show that synthetic siRNA molecules corresponding to the AHSV VP7 gene silenced effectively VP7 protein and mRNA expression, and decreased production of infectious virus particles as evidenced by a reduction in the progeny virion titres when compared to control cells. This work establishes RNAi as a genetic tool for the study of AHSV and offers new possibilities for the analysis of viral genes important for AHSV physiology. JF - Virus Genes SN - 0920-8569 AD - Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria, 0002, South Africa, lieselburger@tuks.co.za. TI - Silencing of African horse sickness virus VP7 protein expression in cultured cells by RNA interference. KW - african-horse-sickness KW - orbivirus KW - rnai AU - Stassen, Liesel AU - Huismans, Henk AU - Theron, Jacques PY - 2007/09/13/ UR - http://dx.doi.org/10.1007/s11262-007-0162-4 ER -