Efficient repair of genomic double-strand breaks by homologous recombination between directly repeated sequences in the plant genome.

@article{citeulike:2801085, abstract = {Previous studies demonstrated that in somatic plant cells, homologous recombination (HR) is several orders of magnitude less efficient than nonhomologous end joining and that HR is little used for genomic double-strand break (DSB) repair. Here, we provide evidence that if genomic DSBs are induced in close proximity to homologous repeats, they can be repaired in up to one-third of cases by HR in transgenic tobacco. Our findings are relevant for the evolution of plant genomes because they indicate that sequences containing direct repeats such as retroelements might be less stable in plants that harbor active mobile elements than anticipated previously. Furthermore, our experimental setup enabled us to demonstrate that transgenic sequences flanked by sites of a rare cutting restriction enzyme can be excised efficiently from the genome of a higher eukaryote by HR as well as by nonhomologous end joining. This makes DSB-induced recombination an attractive alternative to the currently applied sequence-specific recombination systems used for genome manipulations, such as marker gene excision.}, address = {Institut f\"{u}r Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, D-06466 Gatersleben, Germany.}, author = {Siebert, R. and Puchta, H. }, citeulike-article-id = {2801085}, issn = {1040-4651}, journal = {The Plant cell}, keywords = {dsb\_repair, gene\_conversion}, month = {May}, number = {5}, pages = {1121--1131}, posted-at = {2008-05-15 10:11:03}, priority = {2}, title = {Efficient repair of genomic double-strand breaks by homologous recombination between directly repeated sequences in the plant genome.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12034901}, volume = {14}, year = {2002} }

TY - JOUR ID - citeulike:2801085 L3 - citeulike-article-id:2801085 TI - Efficient repair of genomic double-strand breaks by homologous recombination between directly repeated sequences in the plant genome. JF - The Plant cell VL - 14 IS - 5 SP - 1121 EP - 1131 AD - Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, D-06466 Gatersleben, Germany. SN - 1040-4651 N2 - Previous studies demonstrated that in somatic plant cells, homologous recombination (HR) is several orders of magnitude less efficient than nonhomologous end joining and that HR is little used for genomic double-strand break (DSB) repair. Here, we provide evidence that if genomic DSBs are induced in close proximity to homologous repeats, they can be repaired in up to one-third of cases by HR in transgenic tobacco. Our findings are relevant for the evolution of plant genomes because they indicate that sequences containing direct repeats such as retroelements might be less stable in plants that harbor active mobile elements than anticipated previously. Furthermore, our experimental setup enabled us to demonstrate that transgenic sequences flanked by sites of a rare cutting restriction enzyme can be excised efficiently from the genome of a higher eukaryote by HR as well as by nonhomologous end joining. This makes DSB-induced recombination an attractive alternative to the currently applied sequence-specific recombination systems used for genome manipulations, such as marker gene excision. KW - dsb_repair KW - gene_conversion AU - Siebert, R AU - Puchta, H PY - 2002/05// UR - http://view.ncbi.nlm.nih.gov/pubmed/12034901 ER -