Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena

by: Todd A Black, Yuping Cai, Peter C Wolk

Molecular Microbiology, Vol. 9, No. 1. (1993), pp. 77-84.

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biochempaper, heterocyst, hetr

Abstract

Summary The spatially patterned differentiation of hetero-cysts in the filamentous cyanobacterium Anabaena requires a functional hetR gene. Transcriptional fusions to luxAB show that hetR is transcribed at a low level throughout the filament when Anabaena is grown with combined nitrogen, and that induction of the gene begins within 2 h following nitrogen deprivation. By 3.5 h, induction is localized to spaced foci. By 6h, there is an overall induction of at least threefold in whole cultures, reflecting at least a 20-fold increase within spatially separated cells. The induction requires the presence of a functional hetR gene, indicating that hetR is autoregulatory. Full induction of a heterocyst structural gene, hepA, also requires a functional hetR locus.

@article{citeulike:2773446, abstract = {Summary The spatially patterned differentiation of hetero-cysts in the filamentous cyanobacterium Anabaena requires a functional hetR gene. Transcriptional fusions to luxAB show that hetR is transcribed at a low level throughout the filament when Anabaena is grown with combined nitrogen, and that induction of the gene begins within 2 h following nitrogen deprivation. By 3.5 h, induction is localized to spaced foci. By 6h, there is an overall induction of at least threefold in whole cultures, reflecting at least a 20-fold increase within spatially separated cells. The induction requires the presence of a functional hetR gene, indicating that hetR is autoregulatory. Full induction of a heterocyst structural gene, hepA, also requires a functional hetR locus.}, author = {Black, Todd A. and Cai, Yuping and Wolk, Peter C. }, citeulike-article-id = {2773446}, doi = {10.1111/j.1365-2958.1993.tb01670.x}, journal = {Molecular Microbiology}, keywords = {biochempaper, heterocyst, hetr}, number = {1}, pages = {77--84}, posted-at = {2008-05-08 20:34:21}, priority = {2}, title = {Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena}, url = {http://www.blackwell-synergy.com/doi/abs/10.1111/j.1365-2958.1993.tb01670.x}, volume = {9}, year = {1993} }

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TY - JOUR ID - citeulike:2773446 L3 - citeulike-article-id:2773446 TI - Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena JF - Molecular Microbiology VL - 9 IS - 1 SP - 77 EP - 84 N2 - Summary The spatially patterned differentiation of hetero-cysts in the filamentous cyanobacterium Anabaena requires a functional hetR gene. Transcriptional fusions to luxAB show that hetR is transcribed at a low level throughout the filament when Anabaena is grown with combined nitrogen, and that induction of the gene begins within 2 h following nitrogen deprivation. By 3.5 h, induction is localized to spaced foci. By 6h, there is an overall induction of at least threefold in whole cultures, reflecting at least a 20-fold increase within spatially separated cells. The induction requires the presence of a functional hetR gene, indicating that hetR is autoregulatory. Full induction of a heterocyst structural gene, hepA, also requires a functional hetR locus. KW - biochempaper KW - heterocyst KW - hetr AU - Black, Todd AU - Cai, Yuping AU - Wolk, Peter PY - 1993/// UR - http://www.blackwell-synergy.com/doi/abs/10.1111/j.1365-2958.1993.tb01670.x ER -

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