Potent effect of target structure on microRNA function.

@article{citeulike:1204879, abstract = {MicroRNAs (miRNAs) are small noncoding RNAs that repress protein synthesis by binding to target messenger RNAs. We investigated the effect of target secondary structure on the efficacy of repression by miRNAs. Using structures predicted by the Sfold program, we model the interaction between an miRNA and a target as a two-step hybridization reaction: nucleation at an accessible target site followed by hybrid elongation to disrupt local target secondary structure and form the complete miRNA-target duplex. This model accurately accounts for the sensitivity to repression by let-7 of various mutant forms of the Caenorhabditis elegans lin-41 3' untranslated region and for other experimentally tested miRNA-target interactions in C. elegans and Drosophila melanogaster. These findings indicate a potent effect of target structure on target recognition by miRNAs and establish a structure-based framework for genome-wide identification of animal miRNA targets.}, address = {Wadsworth Center, New York State Department of Health, 150 New Scotland Avenue, Albany, New York 12208, USA.}, author = {Long, Dang and Lee, Rosalind and Williams, Peter and Chan, Chi Yu Y. and Ambros, Victor and Ding, Ye }, citeulike-article-id = {1204879}, doi = {http://dx.doi.org/10.1038/nsmb1226}, issn = {1545-9993}, journal = {Nat Struct Mol Biol}, keywords = {mirna, mirna\_target}, month = {April}, posted-at = {2008-03-30 17:18:10}, priority = {0}, title = {Potent effect of target structure on microRNA function.}, url = {http://dx.doi.org/10.1038/nsmb1226}, year = {2007} }

TY - JOUR ID - citeulike:1204879 L3 - citeulike-article-id:1204879 TI - Potent effect of target structure on microRNA function. JF - Nat Struct Mol Biol AD - Wadsworth Center, New York State Department of Health, 150 New Scotland Avenue, Albany, New York 12208, USA. SN - 1545-9993 N2 - MicroRNAs (miRNAs) are small noncoding RNAs that repress protein synthesis by binding to target messenger RNAs. We investigated the effect of target secondary structure on the efficacy of repression by miRNAs. Using structures predicted by the Sfold program, we model the interaction between an miRNA and a target as a two-step hybridization reaction: nucleation at an accessible target site followed by hybrid elongation to disrupt local target secondary structure and form the complete miRNA-target duplex. This model accurately accounts for the sensitivity to repression by let-7 of various mutant forms of the Caenorhabditis elegans lin-41 3' untranslated region and for other experimentally tested miRNA-target interactions in C. elegans and Drosophila melanogaster. These findings indicate a potent effect of target structure on target recognition by miRNAs and establish a structure-based framework for genome-wide identification of animal miRNA targets. KW - mirna KW - mirna_target AU - Long, Dang AU - Lee, Rosalind AU - Williams, Peter AU - Chan, Chi Yu AU - Ambros, Victor AU - Ding, Ye PY - 2007/04/01/ UR - http://dx.doi.org/10.1038/nsmb1226 ER -