Lin-28 interaction with the Let-7 precursor loop mediates regulated microRNA processing.

@article{citeulike:2925299, abstract = {A hallmark of mammalian embryonic development is the widespread induction of microRNA (miRNA) expression. Surprisingly, the transcription of many of these small, noncoding RNAs is unchanged through development; rather, a post-transcriptional regulatory event prevents accumulation of the mature miRNA species. Here, we present a biochemical framework for the regulated production of the Let-7 family of miRNAs. Embryonic cells contain a Drosha Inhibitor that prevents processing of the Let-7 primary transcript. This inhibitor specifically binds to conserved nucleotides in the loop region of the Let-7 precursor, and competitor RNAs that mimic the binding site restore Let-7 processing. We have identified the Drosha Inhibitor as the embryonic stem cell specific protein Lin-28. Lin-28 has been previously implicated in developmental regulatory pathways in Caenorhabditis elegans, and it promotes reprogramming of human somatic cells into pluripotent stem cells. Our findings outline a microRNA post-transcriptional regulatory network and establish a novel role for the miRNA precursor loop in the regulated production of mature Let-7.}, address = {1Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA.}, author = {Newman, Martin A A. and Thomson, J Michael M. and Hammond, Scott M M. }, citeulike-article-id = {2925299}, doi = {http://dx.doi.org/10.1261/rna.1155108}, issn = {1469-9001}, journal = {RNA (New York, N.Y.)}, keywords = {microrna, microrna\_regulation, post\_transcriptional, processing}, month = {June}, posted-at = {2008-06-25 08:41:32}, priority = {2}, title = {Lin-28 interaction with the Let-7 precursor loop mediates regulated microRNA processing.}, url = {http://dx.doi.org/10.1261/rna.1155108}, year = {2008} }

TY - JOUR ID - citeulike:2925299 L3 - citeulike-article-id:2925299 N2 - A hallmark of mammalian embryonic development is the widespread induction of microRNA (miRNA) expression. Surprisingly, the transcription of many of these small, noncoding RNAs is unchanged through development; rather, a post-transcriptional regulatory event prevents accumulation of the mature miRNA species. Here, we present a biochemical framework for the regulated production of the Let-7 family of miRNAs. Embryonic cells contain a Drosha Inhibitor that prevents processing of the Let-7 primary transcript. This inhibitor specifically binds to conserved nucleotides in the loop region of the Let-7 precursor, and competitor RNAs that mimic the binding site restore Let-7 processing. We have identified the Drosha Inhibitor as the embryonic stem cell specific protein Lin-28. Lin-28 has been previously implicated in developmental regulatory pathways in Caenorhabditis elegans, and it promotes reprogramming of human somatic cells into pluripotent stem cells. Our findings outline a microRNA post-transcriptional regulatory network and establish a novel role for the miRNA precursor loop in the regulated production of mature Let-7. JF - RNA (New York, N.Y.) SN - 1469-9001 AD - 1Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA. TI - Lin-28 interaction with the Let-7 precursor loop mediates regulated microRNA processing. KW - microrna KW - microrna_regulation KW - post_transcriptional KW - processing AU - Newman, Martin A AU - Thomson, J Michael AU - Hammond, Scott M PY - 2008/06/19/ UR - http://dx.doi.org/10.1261/rna.1155108 ER -