Engineering the luxCDABE genes from Photorhabdus luminescens to provide a bioluminescent reporter for constitutive and promoter probe plasmids and mini-Tn5 constructs.

by: MK Winson, S Swift, PJ Hill, CM Sims, G Griesmayr, BW Bycroft, P Williams, GS Stewart

FEMS microbiology letters, Vol. 163, No. 2. (15 June 1998), pp. 193-202.

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Abstract

The luxCDABE operon of Photorhabdus luminescens has been cloned and engineered as an easily mobilisable cassette flanked by sites for commonly used restriction enzymes. Constitutive and promoter probe plasmids utilising the P. luminescens luxCDABE have been constructed using a number of compatible replicons and antibiotic markers. Complementary to these plasmids, a range of promoterless and constitutive luxCDABE mini-Tn5 derivatives has been constructed. The potential of coupling mini-Tn5 luxCDABE promoter probe transposons with automated luminometry and photometry to screen for mutants that exhibit growth phase variation in gene expression is demonstrated.

@article{citeulike:2997278, abstract = {The luxCDABE operon of Photorhabdus luminescens has been cloned and engineered as an easily mobilisable cassette flanked by sites for commonly used restriction enzymes. Constitutive and promoter probe plasmids utilising the P. luminescens luxCDABE have been constructed using a number of compatible replicons and antibiotic markers. Complementary to these plasmids, a range of promoterless and constitutive luxCDABE mini-Tn5 derivatives has been constructed. The potential of coupling mini-Tn5 luxCDABE promoter probe transposons with automated luminometry and photometry to screen for mutants that exhibit growth phase variation in gene expression is demonstrated.}, address = {Food Microbiology Section, School of Biological Sciences, University of Nottingham Leics, USA.}, author = {Winson, M. K. and Swift, S. and Hill, P. J. and Sims, C. M. and Griesmayr, G. and Bycroft, B. W. and Williams, P. and Stewart, G. S. }, citeulike-article-id = {2997278}, issn = {0378-1097}, journal = {FEMS microbiology letters}, keywords = {lux}, month = {June}, number = {2}, pages = {193--202}, posted-at = {2008-07-13 14:29:23}, priority = {2}, title = {Engineering the luxCDABE genes from Photorhabdus luminescens to provide a bioluminescent reporter for constitutive and promoter probe plasmids and mini-Tn5 constructs.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9673022}, volume = {163}, year = {1998} }

RIS record

TY - JOUR ID - citeulike:2997278 L3 - citeulike-article-id:2997278 TI - Engineering the luxCDABE genes from Photorhabdus luminescens to provide a bioluminescent reporter for constitutive and promoter probe plasmids and mini-Tn5 constructs. JF - FEMS microbiology letters VL - 163 IS - 2 SP - 193 EP - 202 AD - Food Microbiology Section, School of Biological Sciences, University of Nottingham Leics, USA. SN - 0378-1097 N2 - The luxCDABE operon of Photorhabdus luminescens has been cloned and engineered as an easily mobilisable cassette flanked by sites for commonly used restriction enzymes. Constitutive and promoter probe plasmids utilising the P. luminescens luxCDABE have been constructed using a number of compatible replicons and antibiotic markers. Complementary to these plasmids, a range of promoterless and constitutive luxCDABE mini-Tn5 derivatives has been constructed. The potential of coupling mini-Tn5 luxCDABE promoter probe transposons with automated luminometry and photometry to screen for mutants that exhibit growth phase variation in gene expression is demonstrated. KW - lux AU - Winson, MK AU - Swift, S AU - Hill, PJ AU - Sims, CM AU - Griesmayr, G AU - Bycroft, BW AU - Williams, P AU - Stewart, GS PY - 1998/06/15/ UR - http://view.ncbi.nlm.nih.gov/pubmed/9673022 ER -

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