Synthesis of Polyester by Means of Genetic Code Reprogramming

by: Atsushi Ohta, Hiroshi Murakami, Eri Higashimura, Hiroaki Suga

Chemistry & Biology, Vol. 14, No. 12. (26 December 2007), pp. 1315-1322.

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Abstract

Summary Here we report the ribosomal polymerization of [alpha]-hydroxy acids by means of genetic code reprogramming. The flexizyme system, a ribozyme-based tRNA acylation tool, was used to reassign individual codons to seven types of [alpha]-hydroxy acids, and then polyesters were synthesized under controls of the reprogrammed genetic code using a reconstituted cell-free translation system. The sequence and length of the polyester segments were specified by the mRNA template, indicating that high-fidelity ribosome expression of polyesters was possible. This work opens a door for the mRNA-directed synthesis of backbone-altered biopolymers.

@article{citeulike:2173789, abstract = {Summary Here we report the ribosomal polymerization of [alpha]-hydroxy acids by means of genetic code reprogramming. The flexizyme system, a ribozyme-based tRNA acylation tool, was used to reassign individual codons to seven types of [alpha]-hydroxy acids, and then polyesters were synthesized under controls of the reprogrammed genetic code using a reconstituted cell-free translation system. The sequence and length of the polyester segments were specified by the mRNA template, indicating that high-fidelity ribosome expression of polyesters was possible. This work opens a door for the mRNA-directed synthesis of backbone-altered biopolymers.}, author = {Ohta, Atsushi and Murakami, Hiroshi and Higashimura, Eri and Suga, Hiroaki }, citeulike-article-id = {2173789}, doi = {10.1016/j.chembiol.2007.10.015}, journal = {Chemistry \& Biology}, keywords = {code, genetic, invitro, neat, syntheticbiology, translation}, month = {December}, number = {12}, pages = {1315--1322}, posted-at = {2007-12-26 23:24:59}, priority = {0}, title = {Synthesis of Polyester by Means of Genetic Code Reprogramming}, url = {http://dx.doi.org/10.1016/j.chembiol.2007.10.015}, volume = {14}, year = {2007} }

RIS record

TY - JOUR ID - citeulike:2173789 L3 - citeulike-article-id:2173789 TI - Synthesis of Polyester by Means of Genetic Code Reprogramming JF - Chemistry & Biology VL - 14 IS - 12 SP - 1315 EP - 1322 N2 - Summary Here we report the ribosomal polymerization of [alpha]-hydroxy acids by means of genetic code reprogramming. The flexizyme system, a ribozyme-based tRNA acylation tool, was used to reassign individual codons to seven types of [alpha]-hydroxy acids, and then polyesters were synthesized under controls of the reprogrammed genetic code using a reconstituted cell-free translation system. The sequence and length of the polyester segments were specified by the mRNA template, indicating that high-fidelity ribosome expression of polyesters was possible. This work opens a door for the mRNA-directed synthesis of backbone-altered biopolymers. KW - code KW - genetic KW - invitro KW - neat KW - syntheticbiology KW - translation AU - Ohta, Atsushi AU - Murakami, Hiroshi AU - Higashimura, Eri AU - Suga, Hiroaki PY - 2007/12/26/ UR - http://dx.doi.org/10.1016/j.chembiol.2007.10.015 ER -

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