28-Way vertebrate alignment and conservation track in the UCSC Genome Browser

@article{miller07, abstract = {This article describes a set of alignments of 28 vertebrate genome sequences that is provided by the UCSC Genome Browser. The alignments can be viewed on the Human Genome Browser (March 2006 assembly) at http://genome.ucsc.edu, downloaded in bulk by anonymous FTP from http://hgdownload.cse.ucsc.edu/goldenPath/hg18/multiz28way, or analyzed with the Galaxy server at http://g2.bx.psu.edu. This article illustrates the power of this resource for exploring vertebrate and mammalian evolution, using three examples. First, we present several vignettes involving insertions and deletions within protein-coding regions, including a look at some human-specific indels. Then we study the extent to which start codons and stop codons in the human sequence are conserved in other species, showing that start codons are in general more poorly conserved than stop codons. Finally, an investigation of the phylogenetic depth of conservation for several classes of functional elements in the human genome reveals striking differences in the rates and modes of decay in alignability. Each functional class has a distinctive period of stringent constraint, followed by decays that allow (for the case of regulatory regions) or reject (for coding regions and ultraconserved elements) insertions and deletions. 10.1101/gr.6761107}, author = {Miller, Webb and Rosenbloom, Kate and Hardison, Ross C. and Hou, Minmei and Taylor, James and Raney, Brian and Burhans, Richard and King, David C. and Baertsch, Robert and Blankenberg, Daniel and Kosakovsky and Nekrutenko, Anton and Giardine, Belinda and Harris, Robert S. and Tyekucheva, Svitlana and Diekhans, Mark and Pringle, Thomas H. and Murphy, William J. and Lesk, Arthur and Weinstock, George M. and Toh, Kerstin L. and Gibbs, Richard A. and Lander, Eric S. and Siepel, Adam and Haussler, David and Kent, James W. }, citeulike-article-id = {1873083}, doi = {http://dx.doi.org/10.1101/gr.6761107}, journal = {Genome Res.}, keywords = {alignment, comparative\_genomics, ucsc}, month = {November}, pages = {gr.6761107+}, posted-at = {2007-12-11 14:46:01}, priority = {2}, title = {28-Way vertebrate alignment and conservation track in the UCSC Genome Browser}, url = {http://dx.doi.org/10.1101/gr.6761107}, year = {2007} }

TY - JOUR ID - miller07 L3 - citeulike-article-id:1873083 N2 - This article describes a set of alignments of 28 vertebrate genome sequences that is provided by the UCSC Genome Browser. The alignments can be viewed on the Human Genome Browser (March 2006 assembly) at http://genome.ucsc.edu, downloaded in bulk by anonymous FTP from http://hgdownload.cse.ucsc.edu/goldenPath/hg18/multiz28way, or analyzed with the Galaxy server at http://g2.bx.psu.edu. This article illustrates the power of this resource for exploring vertebrate and mammalian evolution, using three examples. First, we present several vignettes involving insertions and deletions within protein-coding regions, including a look at some human-specific indels. Then we study the extent to which start codons and stop codons in the human sequence are conserved in other species, showing that start codons are in general more poorly conserved than stop codons. Finally, an investigation of the phylogenetic depth of conservation for several classes of functional elements in the human genome reveals striking differences in the rates and modes of decay in alignability. Each functional class has a distinctive period of stringent constraint, followed by decays that allow (for the case of regulatory regions) or reject (for coding regions and ultraconserved elements) insertions and deletions. 10.1101/gr.6761107 JF - Genome Res. TI - 28-Way vertebrate alignment and conservation track in the UCSC Genome Browser SP - gr.6761107 KW - alignment KW - comparative_genomics KW - ucsc AU - Miller, Webb AU - Rosenbloom, Kate AU - Hardison, Ross AU - Hou, Minmei AU - Taylor, James AU - Raney, Brian AU - Burhans, Richard AU - King, David AU - Baertsch, Robert AU - Blankenberg, Daniel AU - Kosakovsky AU - Nekrutenko, Anton AU - Giardine, Belinda AU - Harris, Robert AU - Tyekucheva, Svitlana AU - Diekhans, Mark AU - Pringle, Thomas AU - Murphy, William AU - Lesk, Arthur AU - Weinstock, George AU - Toh, Kerstin AU - Gibbs, Richard AU - Lander, Eric AU - Siepel, Adam AU - Haussler, David AU - Kent, James PY - 2007/November/05/ UR - http://dx.doi.org/10.1101/gr.6761107 ER -