Remote control of gene expression.

@article{long07, abstract = {The elucidation of a growing number of species' genomes heralds an unprecedented opportunity to ascertain functional attributes of non-coding sequences. In particular, cis regulatory modules (CRMs) controlling gene expression constitute a rich treasure trove of data to be defined and experimentally validated. Such information will provide insight into cell lineage determination and differentiation and the genetic basis of heritable diseases as well as the development of novel tools for restricting the inactivation of genes to specific cell types or conditions. Historically, the study of CRMs and their individual transcription factor binding sites has been limited to proximal regions around gene loci. Two important by-products of the genomics revolution, artificial chromosome vectors and comparative genomics, have fueled efforts to define an increasing number of CRMs acting remotely to control gene expression. Such regulation from a distance has challenged our perspectives of gene expression control and perhaps the very definition of a gene. This review summarizes current approaches to characterize remote control of gene expression in transgenic mice and inherent limitations for accurately interpreting the essential nature of CRM activity.}, address = {Cardiovascular Research Institute, University of Rochester School of Medicine, Rochester, New York 14642, USA.}, author = {Long, X. and Miano, J. M. }, citeulike-article-id = {1558155}, doi = {http://dx.doi.org/10.1074/jbc.R700010200}, issn = {0021-9258}, journal = {J Biol Chem}, keywords = {cns, comparative\_genomics, regulatory}, month = {June}, number = {22}, pages = {15941--15945}, posted-at = {2007-08-13 16:33:48}, priority = {2}, title = {Remote control of gene expression.}, url = {http://dx.doi.org/10.1074/jbc.R700010200}, volume = {282}, year = {2007} }

TY - JOUR ID - long07 L3 - citeulike-article-id:1558155 N2 - The elucidation of a growing number of species' genomes heralds an unprecedented opportunity to ascertain functional attributes of non-coding sequences. In particular, cis regulatory modules (CRMs) controlling gene expression constitute a rich treasure trove of data to be defined and experimentally validated. Such information will provide insight into cell lineage determination and differentiation and the genetic basis of heritable diseases as well as the development of novel tools for restricting the inactivation of genes to specific cell types or conditions. Historically, the study of CRMs and their individual transcription factor binding sites has been limited to proximal regions around gene loci. Two important by-products of the genomics revolution, artificial chromosome vectors and comparative genomics, have fueled efforts to define an increasing number of CRMs acting remotely to control gene expression. Such regulation from a distance has challenged our perspectives of gene expression control and perhaps the very definition of a gene. This review summarizes current approaches to characterize remote control of gene expression in transgenic mice and inherent limitations for accurately interpreting the essential nature of CRM activity. IS - 22 JF - J Biol Chem SN - 0021-9258 AD - Cardiovascular Research Institute, University of Rochester School of Medicine, Rochester, New York 14642, USA. EP - 15945 TI - Remote control of gene expression. VL - 282 SP - 15941 KW - cns KW - comparative_genomics KW - regulatory AU - Long, X AU - Miano, JM PY - 2007/June/01/ UR - http://dx.doi.org/10.1074/jbc.R700010200 ER -