Searching for markers of Creutzfeldt-Jakob disease in cerebrospinal fluid by two-dimensional mapping.

@article{citeulike:2878398, abstract = {Differential proteomic analysis has been performed on the cerebrospinal fluid (CSF) of six healthy and six patients suffering form sporadic Creutzfeldt-Jakob disease (sCJD), age- and sex-matched, after immuno-subtraction of albumin and immunoglobulins. These maps have revealed 28 polypeptide chains differentially modulated in the sCJD samples, of which 10 appeared to be up-regulated, the remaining 18 being down-regulated. Among those, 13 could be identified upon digestion and MALDI-TOF, MS analysis. In addition, the strong modulation of cystatin C was also confirmed by immunoblot analysis and the highly altered level of the 14-3-3 proteins that escaped detection by 2-D mapping, could be assessed by Western blots and immuno-detection of monomeric and homo- and hetero-dimeric 14-3-3 isotypes. In search for a panel of potential markers for sCJD, we highlight cystatin C, 14-3-3 proteins, transferrin, ubiquitin, Apoliprotein J and perhaps some of the still unidentified, but strongly modulated polypeptide chains detected in the differential map.}, address = {Department of Neurological and Visual Sciences, Section of Neurology, University of Verona, Verona, Italy.}, author = {Piubelli, C. and Fiorini, M. and Zanusso, G. and Milli, A. and Fasoli, E. and Monaco, S. and Righetti, P. G. }, citeulike-article-id = {2878398}, doi = {http://dx.doi.org/10.1002/pmic.200500294}, issn = {1615-9861}, journal = {Proteomics}, keywords = {cjd, csf}, month = {April}, posted-at = {2008-06-10 01:57:40}, priority = {5}, title = {Searching for markers of Creutzfeldt-Jakob disease in cerebrospinal fluid by two-dimensional mapping.}, url = {http://dx.doi.org/10.1002/pmic.200500294}, volume = {6 Suppl 1}, year = {2006} }

TY - JOUR ID - citeulike:2878398 L3 - citeulike-article-id:2878398 N2 - Differential proteomic analysis has been performed on the cerebrospinal fluid (CSF) of six healthy and six patients suffering form sporadic Creutzfeldt-Jakob disease (sCJD), age- and sex-matched, after immuno-subtraction of albumin and immunoglobulins. These maps have revealed 28 polypeptide chains differentially modulated in the sCJD samples, of which 10 appeared to be up-regulated, the remaining 18 being down-regulated. Among those, 13 could be identified upon digestion and MALDI-TOF, MS analysis. In addition, the strong modulation of cystatin C was also confirmed by immunoblot analysis and the highly altered level of the 14-3-3 proteins that escaped detection by 2-D mapping, could be assessed by Western blots and immuno-detection of monomeric and homo- and hetero-dimeric 14-3-3 isotypes. In search for a panel of potential markers for sCJD, we highlight cystatin C, 14-3-3 proteins, transferrin, ubiquitin, Apoliprotein J and perhaps some of the still unidentified, but strongly modulated polypeptide chains detected in the differential map. JF - Proteomics SN - 1615-9861 AD - Department of Neurological and Visual Sciences, Section of Neurology, University of Verona, Verona, Italy. TI - Searching for markers of Creutzfeldt-Jakob disease in cerebrospinal fluid by two-dimensional mapping. VL - 6 Suppl 1 KW - cjd KW - csf AU - Piubelli, C AU - Fiorini, M AU - Zanusso, G AU - Milli, A AU - Fasoli, E AU - Monaco, S AU - Righetti, PG PY - 2006/04// UR - http://dx.doi.org/10.1002/pmic.200500294 ER -