Divergence of Transcription Factor Binding Sites Across Related Yeast Species

by: Anthony R Borneman, Tara A Gianoulis, Zhengdong D Zhang, Haiyuan Yu, Joel Rozowsky, Michael R Seringhaus, Lu Y Wang, Mark Gerstein, Michael Snyder

Science, Vol. 317, No. 5839. (10 August 2007), pp. 815-819.

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Abstract

Characterization of interspecies differences in gene regulation is crucial for understanding the molecular basis of both phenotypic diversity and evolution. By means of chromatin immunoprecipitation and DNA microarray analysis, the divergence in the binding sites of the pseudohyphal regulators Ste12 and Tec1 was determined in the yeasts Saccharomyces cerevisiae, S. mikatae, and S. bayanus under pseudohyphal conditions. We have shown that most of these sites have diverged across these species, far exceeding the interspecies variation in orthologous genes. A group of Ste12 targets was shown to be bound only in S. mikatae and S. bayanus under pseudohyphal conditions. Many of these genes are targets of Ste12 during mating in S. cerevisiae, indicating that specialization between the two pathways has occurred in this species. Transcription factor binding sites have therefore diverged substantially faster than ortholog content. Thus, gene regulation resulting from transcription factor binding is likely to be a major cause of divergence between related species. 10.1126/science.1140748

@article{citeulike:1551105, abstract = {Characterization of interspecies differences in gene regulation is crucial for understanding the molecular basis of both phenotypic diversity and evolution. By means of chromatin immunoprecipitation and DNA microarray analysis, the divergence in the binding sites of the pseudohyphal regulators Ste12 and Tec1 was determined in the yeasts Saccharomyces cerevisiae, S. mikatae, and S. bayanus under pseudohyphal conditions. We have shown that most of these sites have diverged across these species, far exceeding the interspecies variation in orthologous genes. A group of Ste12 targets was shown to be bound only in S. mikatae and S. bayanus under pseudohyphal conditions. Many of these genes are targets of Ste12 during mating in S. cerevisiae, indicating that specialization between the two pathways has occurred in this species. Transcription factor binding sites have therefore diverged substantially faster than ortholog content. Thus, gene regulation resulting from transcription factor binding is likely to be a major cause of divergence between related species. 10.1126/science.1140748}, author = {Borneman, Anthony R. and Gianoulis, Tara A. and Zhang, Zhengdong D. and Yu, Haiyuan and Rozowsky, Joel and Seringhaus, Michael R. and Wang, Lu Y. and Gerstein, Mark and Snyder, Michael }, citeulike-article-id = {1551105}, doi = {10.1126/science.1140748}, journal = {Science}, month = {August}, number = {5839}, pages = {815--819}, posted-at = {2008-04-28 08:17:44}, priority = {2}, title = {Divergence of Transcription Factor Binding Sites Across Related Yeast Species}, url = {http://dx.doi.org/10.1126/science.1140748}, volume = {317}, year = {2007} }

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TY - JOUR ID - citeulike:1551105 L3 - citeulike-article-id:1551105 TI - Divergence of Transcription Factor Binding Sites Across Related Yeast Species JF - Science VL - 317 IS - 5839 SP - 815 EP - 819 N2 - Characterization of interspecies differences in gene regulation is crucial for understanding the molecular basis of both phenotypic diversity and evolution. By means of chromatin immunoprecipitation and DNA microarray analysis, the divergence in the binding sites of the pseudohyphal regulators Ste12 and Tec1 was determined in the yeasts Saccharomyces cerevisiae, S. mikatae, and S. bayanus under pseudohyphal conditions. We have shown that most of these sites have diverged across these species, far exceeding the interspecies variation in orthologous genes. A group of Ste12 targets was shown to be bound only in S. mikatae and S. bayanus under pseudohyphal conditions. Many of these genes are targets of Ste12 during mating in S. cerevisiae, indicating that specialization between the two pathways has occurred in this species. Transcription factor binding sites have therefore diverged substantially faster than ortholog content. Thus, gene regulation resulting from transcription factor binding is likely to be a major cause of divergence between related species. 10.1126/science.1140748 AU - Borneman, Anthony AU - Gianoulis, Tara AU - Zhang, Zhengdong AU - Yu, Haiyuan AU - Rozowsky, Joel AU - Seringhaus, Michael AU - Wang, Lu AU - Gerstein, Mark AU - Snyder, Michael PY - 2007/08/10/ UR - http://dx.doi.org/10.1126/science.1140748 ER -

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