Reg-II is an exocrine pancreas injury-response product that is up-regulated by keratin absence or mutation.

@article{citeulike:3020694, abstract = {The major keratins in the pancreas and liver are keratins 8 and 18 (K8/K18), but their function seemingly differs in that liver K8/K18 are essential cytoprotective proteins, whereas pancreatic K8/K18 are dispensable. This functional dichotomy raises the hypothesis that K8-null pancreata may undergo compensatory cytoprotective gene expression. We tested this hypothesis by comparing the gene expression profile in pancreata of wild-type and K8-null mice. Most prominent among the up-regulated genes in K8-null pancreas was mRNA for regenerating islet-derived (Reg)-II, which was confirmed by quantitative reverse transcription-polymerase chain reaction and by an anti-Reg-II peptide antibody we generated. Both K8-null and wild-type mice express Reg-II predominantly in acinar cells as determined by in situ hybridization and immunostaining. Analysis of Reg-II expression in various keratin-related transgenic mouse models showed that its induction also occurs in response to keratin cytoplasmic filament collapse, absence, or ablation of K18 Ser52 but not Ser33 phosphorylation via Ser-to-Ala mutation, which represent situations associated with predisposition to liver but not pancreatic injury. In wild-type mice, Reg-II is markedly up-regulated in two established pancreatitis models in response to injury and during the recovery phase. Thus, Reg-II is a likely mouse exocrine pancreas cytoprotective candidate protein whose expression is regulated by keratin filament organization and phosphorylation.}, address = {Department of Medicine, Palo Alto Veterans Affairs Medical Center, Palo Alto, CA 94304, USA.}, author = {Zhong, B. and Strnad, P. and Toivola, D. M. and Tao, G. Z. and Ji, X. and Greenberg, H. B. and Omary, M. B. }, citeulike-article-id = {3020694}, doi = {http://dx.doi.org/10.1091/mbc.E07-02-0180}, issn = {1059-1524}, journal = {Molecular biology of the cell}, keywords = {pap}, month = {December}, number = {12}, pages = {4969--4978}, posted-at = {2008-07-19 09:38:03}, priority = {2}, title = {Reg-II is an exocrine pancreas injury-response product that is up-regulated by keratin absence or mutation.}, url = {http://dx.doi.org/10.1091/mbc.E07-02-0180}, volume = {18}, year = {2007} }

TY - JOUR ID - citeulike:3020694 L3 - citeulike-article-id:3020694 TI - Reg-II is an exocrine pancreas injury-response product that is up-regulated by keratin absence or mutation. JF - Molecular biology of the cell VL - 18 IS - 12 SP - 4969 EP - 4978 AD - Department of Medicine, Palo Alto Veterans Affairs Medical Center, Palo Alto, CA 94304, USA. SN - 1059-1524 N2 - The major keratins in the pancreas and liver are keratins 8 and 18 (K8/K18), but their function seemingly differs in that liver K8/K18 are essential cytoprotective proteins, whereas pancreatic K8/K18 are dispensable. This functional dichotomy raises the hypothesis that K8-null pancreata may undergo compensatory cytoprotective gene expression. We tested this hypothesis by comparing the gene expression profile in pancreata of wild-type and K8-null mice. Most prominent among the up-regulated genes in K8-null pancreas was mRNA for regenerating islet-derived (Reg)-II, which was confirmed by quantitative reverse transcription-polymerase chain reaction and by an anti-Reg-II peptide antibody we generated. Both K8-null and wild-type mice express Reg-II predominantly in acinar cells as determined by in situ hybridization and immunostaining. Analysis of Reg-II expression in various keratin-related transgenic mouse models showed that its induction also occurs in response to keratin cytoplasmic filament collapse, absence, or ablation of K18 Ser52 but not Ser33 phosphorylation via Ser-to-Ala mutation, which represent situations associated with predisposition to liver but not pancreatic injury. In wild-type mice, Reg-II is markedly up-regulated in two established pancreatitis models in response to injury and during the recovery phase. Thus, Reg-II is a likely mouse exocrine pancreas cytoprotective candidate protein whose expression is regulated by keratin filament organization and phosphorylation. KW - pap AU - Zhong, B AU - Strnad, P AU - Toivola, DM AU - Tao, GZ AU - Ji, X AU - Greenberg, HB AU - Omary, MB PY - 2007/12// UR - http://dx.doi.org/10.1091/mbc.E07-02-0180 ER -