Solvent-Free Matrix Dry-Coating for MALDI Imaging of Phospholipids.

@article{citeulike:2625300, abstract = {A fast and simple, solvent-free matrix deposition protocol was developed for positive ionization mode phospholipid analysis in tissues. Finely ground 2,5-dihydroxybenzoic acid was deposited onto sagittal mouse brain sections using a dry-coating technique, in which solid matrix particles were filtered directly onto the tissue through a 20-mum stainless steel sieve. Phospholipid signals were obtained directly off these sections, allowing acquisition of high-resolution MS images. These images were compared to those from serial sections that were spray-coated with a thin-layer chromatography (TLC) reagent sprayer. Signals obtained from the dry matrix deposition method were comparable to those from spray-coated sections, producing identical localization patterns with a simpler and faster sample preparation with virtually no analyte delocalization. This approach was found to yield highly reproducible results, eliminating much of the variance caused by operator differences, and making it an attractive alternative to the currently used matrix application methods.}, address = {Department of Chemistry, Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee, USA.}, author = {Puolitaival, Satu M M. and Burnum, Kristin E E. and Cornett, D Shannon S. and Caprioli, Richard M M. }, citeulike-article-id = {2625300}, doi = {http://dx.doi.org/10.1016/j.jasms.2008.02.013}, issn = {1044-0305}, journal = {Journal of the American Society for Mass Spectrometry}, keywords = {imaging, maldi}, month = {March}, posted-at = {2008-04-03 08:12:10}, priority = {0}, title = {Solvent-Free Matrix Dry-Coating for MALDI Imaging of Phospholipids.}, url = {http://dx.doi.org/10.1016/j.jasms.2008.02.013}, year = {2008} }

TY - JOUR ID - citeulike:2625300 L3 - citeulike-article-id:2625300 N2 - A fast and simple, solvent-free matrix deposition protocol was developed for positive ionization mode phospholipid analysis in tissues. Finely ground 2,5-dihydroxybenzoic acid was deposited onto sagittal mouse brain sections using a dry-coating technique, in which solid matrix particles were filtered directly onto the tissue through a 20-mum stainless steel sieve. Phospholipid signals were obtained directly off these sections, allowing acquisition of high-resolution MS images. These images were compared to those from serial sections that were spray-coated with a thin-layer chromatography (TLC) reagent sprayer. Signals obtained from the dry matrix deposition method were comparable to those from spray-coated sections, producing identical localization patterns with a simpler and faster sample preparation with virtually no analyte delocalization. This approach was found to yield highly reproducible results, eliminating much of the variance caused by operator differences, and making it an attractive alternative to the currently used matrix application methods. JF - Journal of the American Society for Mass Spectrometry SN - 1044-0305 AD - Department of Chemistry, Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee, USA. TI - Solvent-Free Matrix Dry-Coating for MALDI Imaging of Phospholipids. KW - imaging KW - maldi AU - Puolitaival, Satu M AU - Burnum, Kristin E AU - Cornett, D Shannon AU - Caprioli, Richard M PY - 2008/03/06/ UR - http://dx.doi.org/10.1016/j.jasms.2008.02.013 ER -