Monitoring mouse prostate development by profiling and imaging mass spectrometry.

@article{citeulike:2609278, abstract = {Mass spectrometry-based tissue profiling and imaging are technologies that allow identification and visualization of protein signals directly on thin sections cut from fresh frozen tissue specimens. These technologies were utilized to evaluate protein expression profiles in the normal mouse prostate during development (1-5 weeks of age), at sexual maturation (6 weeks of age), and in adult prostate (at 10, 15, or 40 weeks of age). The evolution of protein expression during normal prostate development and maturation were subsequently compared with 15-week prostate tumors derived from genetically engineered mice carrying the Large T antigen gene under regulation of the prostate-specific probasin promoter (LPB-Tag mouse model for prostate cancer). This approach identified proteins differentially expressed at specific time points during prostate development. Furthermore expression of some of these proteins, for example probasin and spermine-binding protein, were associated with prostate maturation, and prostate tumor formation resulted in their loss of expression. Cyclophilin A, a protein found in other cancers, was differentially alpha-acetylated on the N terminus, and both isoforms appeared during normal prostate and prostate tumor development. Imaging mass spectrometry localized the protein signals to specific prostatic lobes or regions. Thus, tissue profiling and imaging can be utilized to analyze the ontogeny of protein expression during prostate morphogenesis and tumorigenesis and identify proteins that could potentially serve as biomarkers for prostate cancer.}, address = {Mass Spectrometry Research Center and Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37232-8575, USA.}, author = {Chaurand, P. and Rahman, M. A. and Hunt, T. and Mobley, J. A. and Gu, G. and Latham, J. C. and Caprioli, R. M. and Kasper, S. }, citeulike-article-id = {2609278}, doi = {http://dx.doi.org/10.1074/mcp.M700190-MCP200}, issn = {1535-9484}, journal = {Mol Cell Proteomics}, keywords = {imaging, maldi}, month = {February}, number = {2}, pages = {411--423}, posted-at = {2008-03-28 19:55:45}, priority = {5}, title = {Monitoring mouse prostate development by profiling and imaging mass spectrometry.}, url = {http://dx.doi.org/10.1074/mcp.M700190-MCP200}, volume = {7}, year = {2008} }

TY - JOUR ID - citeulike:2609278 L3 - citeulike-article-id:2609278 N2 - Mass spectrometry-based tissue profiling and imaging are technologies that allow identification and visualization of protein signals directly on thin sections cut from fresh frozen tissue specimens. These technologies were utilized to evaluate protein expression profiles in the normal mouse prostate during development (1-5 weeks of age), at sexual maturation (6 weeks of age), and in adult prostate (at 10, 15, or 40 weeks of age). The evolution of protein expression during normal prostate development and maturation were subsequently compared with 15-week prostate tumors derived from genetically engineered mice carrying the Large T antigen gene under regulation of the prostate-specific probasin promoter (LPB-Tag mouse model for prostate cancer). This approach identified proteins differentially expressed at specific time points during prostate development. Furthermore expression of some of these proteins, for example probasin and spermine-binding protein, were associated with prostate maturation, and prostate tumor formation resulted in their loss of expression. Cyclophilin A, a protein found in other cancers, was differentially alpha-acetylated on the N terminus, and both isoforms appeared during normal prostate and prostate tumor development. Imaging mass spectrometry localized the protein signals to specific prostatic lobes or regions. Thus, tissue profiling and imaging can be utilized to analyze the ontogeny of protein expression during prostate morphogenesis and tumorigenesis and identify proteins that could potentially serve as biomarkers for prostate cancer. IS - 2 JF - Mol Cell Proteomics SN - 1535-9484 AD - Mass Spectrometry Research Center and Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37232-8575, USA. EP - 423 TI - Monitoring mouse prostate development by profiling and imaging mass spectrometry. VL - 7 SP - 411 KW - imaging KW - maldi AU - Chaurand, P AU - Rahman, MA AU - Hunt, T AU - Mobley, JA AU - Gu, G AU - Latham, JC AU - Caprioli, RM AU - Kasper, S PY - 2008/02// UR - http://dx.doi.org/10.1074/mcp.M700190-MCP200 ER -